Ectopic overexpression of a novel Glycine soja stress-induced plasma membrane intrinsic protein increases sensitivity to salt and dehydration in transgenic Arabidopsis thaliana plants

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• 作者：Xi Wang ; Hua Cai ; Yong Li ; Yanming Zhu ; Wei Ji ; Xi Bai…
• 关键词：Abiotic stress tolerance ; Aquaporin (AQP) ; Arabidopsis thaliana ; Glycine soja ; Major intrinsic protein (MIP) ; Plasma membrane intrinsic protein (PIP)
• 刊名：Journal of Plant Research
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：128
• 期：1
• 页码：103-113
• 全文大小：7,924 KB
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• 作者单位：Xi Wang (1) (2)
  Hua Cai (3)
  Yong Li (3)
  Yanming Zhu (3)
  Wei Ji (3)
  Xi Bai (3)
  Dan Zhu (3)
  Xiaoli Sun (3)
  
  1. The Key Laboratory of Sugar Beet Genetic Breeding, Colleges of Heilongjiang Province, Heilongjiang University, No. 74 XueFu Road, NanGang District, Harbin, 150080, Heilongjiang, China
  2. Sugar Beet Research Institute of Chinese Academy of Agricultural Sciences, Crop Academy of Heilongjiang University, No. 74 XueFu Road, NanGang District, Harbin, 150080, Heilongjiang, China
  3. Plant Bioengineering Laboratory, Northeast Agricultural University, No. 59 MuCai Street, XiangFang District, Harbin, 150030, Heilongjiang, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Ecology
  Plant Physiology
  Plant Biochemistry
  
• 出版者：Springer Japan
• ISSN：1618-0860

文摘

Plasma membrane intrinsic proteins (PIPs) belong to the aquaporin family and facilitate water movement across plasma membranes. Existing data indicate that PIP genes are associated with the abilities of plants to tolerate certain stress conditions. A review of our Glycine soja expressed sequence tag (EST) dataset revealed that abiotic stress stimulated expression of a PIP, herein designated as GsPIP2;1 (GenBank_Accn: FJ825766). To understand the roles of this PIP in stress tolerance, we generated a coding sequence for GsPIP2;1 by in silico elongation and cloned the cDNA by 5-RACE. Semiquantitative RT-PCR showed that GsPIP2;1 expression was stimulated in G. soja leaves by cold, salt, or dehydration stress, whereas the same stresses suppressed GsPIP2;1 expression in the roots. Transgenic Arabidopsis thaliana plants overexpressing GsPIP2;1 grew normally under unstressed and cold conditions, but exhibited depressed tolerance to salt and dehydration stresses. Moreover, greater changes in water potential were detected in the transgenic A. thaliana shoots, implying that GsPIP2;1 may negatively impact stress tolerance by regulating water potential. These results, deviating from those obtained in previous reports, provide new insights into the relationship between PIPs and abiotic stress tolerance in plants.