TsMIP6 enhances the tolerance of transgenic rice to salt stress and interacts with target proteins

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• 作者：Linlin Sun ; Guohong Yu ; Xiaori Han ; Shichao Xin ; Xiaojing Qiang…
• 关键词：Aquaporin ; Interaction ; Real ; time PCR ; Salt stress ; Transgenic Kitaake
• 刊名：Journal of Plant Biology
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：58
• 期：5
• 页码：285-292
• 全文大小：1,704 KB
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• 作者单位：Linlin Sun (1) (2)
  Guohong Yu (2)
  Xiaori Han (1)
  Shichao Xin (2) (3)
  Xiaojing Qiang (2)
  Linlin Jiang (2)
  Shuhui Zhang (1) (2)
  Xianguo Cheng (2)
  
  1. College of Land and Environment, Shenyang Agricultural University, No. 120 Dongling Road, Shenyang, Liaoning, 110866, PR China
  2. Key Lab of Plant Nutrition and Fertilizers, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, No. 12 South Street, Zhongguancun, Beijing, 100081, PR China
  3. Key Laboratory of Rubber Biology, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan, 571737, PR China
  
• 刊物主题：Plant Sciences; Plant Breeding/Biotechnology; Plant Genetics & Genomics; Plant Systematics/Taxonomy/Biogeography; Plant Ecology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1867-0725

文摘

Aquaporins (AQPs), a large family of channel proteins in plants, play an important role in regulating the balance of osmotic potential in cells. We isolated an AQP gene, TsMIP6, from the halophyte Thellungiella salsuginea and functionally characterized it in transgenic rice (Oryza sativa). This gene belongs to a subfamily of tonoplast intrinsic proteins and is localized at the plasma membrane. Real-time PCR showed that expression of TsMIP6 in shoots or roots of T. salsuginea was markedly induced by salinity, whereas its ectopic expression in ‘Kitaake-lines of rice significantly increased plant tolerance to salt stress. Physiological data suggested that TsMIP6 is involved in regulating ion homeostasis and water channel activity in salt-stressed transgenic rice. Heterologous expression analysis indicated that TsMIP6 specifically interacts with a member of the glycoside hydrolase family 64 protein #617 in yeast cells. This suggests that the relationship between TsMIP6 and #617 has a crucial role in mediating osmotic balance in plant cells. Moreover, TsMIP6 might help to modulate the transport of some neutral molecules and may function through a pathway regulating solute equilibrium to maintain osmotic potential. Keywords Aquaporin Interaction Real-time PCR Salt stress Transgenic Kitaake