Salicylic acid treatment enhances expression of chalcone isomerase gene and accumulation of corresponding flavonoids during fruit maturation of Lycium chinense

详细信息    查看全文

• 作者：Chunfeng Guan (1)
  Xinyu Song (2)
  Jing Ji (1)
  Xiaozhou Li (3)
  Chao Jin (1)
  Wenzhu Guan (2)
  Jing Li (1)
  Gang Wang (1)
  
• 关键词：Chalcone isomerase ; Flavonoid ; Fruit ; Lycium chinense ; Salicylic acid
• 刊名：European Food Research and Technology
• 出版年：2014
• 出版时间：November 2014
• 年：2014
• 卷：239
• 期：5
• 页码：857-865
• 全文大小：1,038 KB
• 参考文献：1. Amagase H, Farnsworth NR (2011) A review of botanical characteristics, phytochemistry, clinical relevance in efficacy and safety of / Lycium barbarum fruit (Goji). Food Res Int 44(7):1702鈥?717 CrossRef
  2. Gan L, Hua Zhang S, Liang Yang X, Bi Xu H (2004) Immunomodulation and antitumor activity by a polysaccharide鈥損rotein complex from / Lycium barbarum. Int Immunopharmacol 4(4):563鈥?69 CrossRef
  3. Ha K-T, Yoon S-J, Choi D-Y, Kim D-W, Kim J-K, Kim C-H (2005) Protective effect of / Lycium chinense fruit on carbon tetrachloride-induced hepatotoxicity. J Ethnopharmacol 96(3):529鈥?35 CrossRef
  4. Chahar MK, Sharma N, Dobhal MP, Joshi YC (2011) Flavonoids: a versatile source of anticancer drugs. Pharmacogn Rev 5(9):1 CrossRef
  5. Ghasemzadeh A, Ghasemzadeh N (2011) Flavonoids and phenolic acids: role and biochemical activity in plants and human. J Med Plants Res 5(31):6697鈥?703
  6. Park SJ, Myoung H, Kim YY, Paeng JY, Park JW, Kim MJ, Hong SM (2008) Anticancer effects of genistein, green tea catechins, and cordycepin on oral squamous cell carcinoma. J Korean Assoc Oral Maxillofac Surg 34(1):1鈥?0
  7. Williams CA, Grayer RJ (2004) Anthocyanins and other flavonoids. Nat Prod Rep 21(4):539鈥?73 CrossRef
  8. Winkel-Shirley B (2001) Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology, and biotechnology. Plant Physiol 126(2):485鈥?93 CrossRef
  9. Liang N-N, He F, Bi H-Q, Duan C-Q, Reeves M, Wang J (2012) Evolution of flavonols in berry skins of different grape cultivars during ripening and a comparison of two vintages. Eur Food Res Technol 235(6):1187鈥?197 CrossRef
  10. Li F, Jin Z, Qu W, Zhao D, Ma F (2006) Cloning of a cDNA encoding the / Saussurea medusa chalcone isomerase and its expression in transgenic tobacco. Plant Physiol Biochem 44(7):455鈥?61 CrossRef
  11. Druka A, Kudrna D, Rostoks N, Brueggeman R, von Wettstein D, Kleinhofs A (2003) Chalcone isomerase gene from rice ( / Oryza sativa) and barley ( / Hordeum vulgare): physical, genetic and mutation mapping. Gene 302(1):171鈥?78 CrossRef
  12. McKhann HI, Hirsch AM (1994) Isolation of chalcone synthase and chalcone isomerase cDNAs from alfalfa ( / Medicago sativa L.): highest transcript levels occur in young roots and root tips. Plant Mol Biol 24(5):767鈥?77 CrossRef
  13. Rivas-San Vicente M, Plasencia J (2011) Salicylic acid beyond defence: its role in plant growth and development. J Exp Bot 62(10):3321鈥?338 CrossRef
  14. Hayat Q, Hayat S, Irfan M, Ahmad A (2010) Effect of exogenous salicylic acid under changing environment: a review. Environ Exp Bot 68(1):14鈥?5 CrossRef
  15. Gechev T, Gadjev I, Van Breusegem F, Inz茅 D, Dukiandjiev S, Toneva V, Minkov I (2002) Hydrogen peroxide protects tobacco from oxidative stress by inducing a set of antioxidant enzymes. Cell Mol Life Sci 59(4):708鈥?14 CrossRef
  16. Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7(9):405鈥?10 CrossRef
  17. Xu M, Dong J, Wang H, Huang L (2009) Complementary action of jasmonic acid on salicylic acid in mediating fungal elicitor-induced flavonol glycoside accumulation of / Ginkgo biloba cells. Plant Cell Environ 32(8):960鈥?67 CrossRef
  18. Campos 脗D, Ferreira AG, Hampe MMV, Antunes IF, Branc茫o N, Silveira EP, da Silva JB, Os贸rio VA (2003) Induction of chalcone synthase and phenylalanine ammonia-lyase by salicylic acid and / Colletotrichum lindemuthianum in common bean. Braz J Plant Physiol 15(3):129鈥?34 CrossRef
  19. Xu F, Cheng H, Cai R, Li LL, Chang J, Zhu J, Zhang FX, Chen LJ, Wang Y, Cheng SH (2008) Molecular cloning and function analysis of an anthocyanidin synthase gene from / Ginkgo biloba, and its expression in abiotic stress responses. Mol Cells 26(6):536鈥?47
  20. Yu Z鈥揨, Fu C-X, Han Y-S, Li Y-X, Zhao D-X (2006) Salicylic acid enhances jaceosidin and syringin production in cell cultures of / Saussurea medusa. Biotechnol Lett 28(13):1027鈥?031 CrossRef
  21. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R et al (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23(21):2947鈥?948 CrossRef
  22. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24(8):1596鈥?599 CrossRef
  23. Sambrook J, Russell D, Russell D (2001) Molecular cloning: a laboratory manual (3 volume set). Cold Spring Harbor Laboratory Press Cold Spring Harbor, New York
  24. Chunfeng G, Xiaozhou L, Gang W, Jing J, Chao J, Josine TL (2013) Expression of cholera toxin B-Lumbrokinase fusion protein in / Pichia pastoris鈥攖he use of transmucosal carriers in the delivery of therapeutic proteins to protect rats against thrombosis. Appl Biochem Biotechnol 169(2):636鈥?50 CrossRef
  25. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1鈥?):248鈥?54 CrossRef
  26. El Hariri B, Sall茅 G, Andary C (1991) Involvement of flavonoids in the resistance of two poplar cultivars to mistletoe ( / Viscum album L.). Protoplasma 162(1):20鈥?6 CrossRef
  27. Dao TTH, Linthorst HJM, Verpoorte R (2011) Chalcone synthase and its functions in plant resistance. Phytochem Rev 10(3):397鈥?12 CrossRef
  28. van Tunen AJ, Hartman SA, Mur LA, Mol JN (1989) Regulation of chalcone flavanone isomerase (CHI) gene expression in / Petunia hybrida: the use of alternative promoters in corolla, anthers and pollen. Plant Mol Biol 12(5):539鈥?51 CrossRef
  29. Wang Y, Li J, Xia R (2010) Expression of chalcone synthase and chalcone isomerase genes and accumulation of corresponding flavonoids during fruit maturation of Guoqing No. 4 satsuma mandarin ( / Citrus unshiu Marcow). Sci Hortic 125(2):110鈥?16 CrossRef
  30. Fang F, Tang K, Huang W-D (2013) Changes of flavonol synthase and flavonol contents during grape berry development. Eur Food Res Technol 237(4):529鈥?40 CrossRef
  31. Wang W, Wang H-L, Wan S-B, Zhang J-H, Zhang P, Zhan J-C, Huang W-D (2012) Chalcone isomerase in grape vine: gene expression and localization in the developing fruit. Biol Plant 56(3):545鈥?50 CrossRef
  32. Smith G, Klages K, Green T, Walton E (1995) Changes in abscisic acid concentration, surface conductance, and water content of developing kiwifruit. Sci Hortic 61(1):13鈥?7 CrossRef
  33. Ochoa M, Kesseler A, Pirone B, M谩rquez C, De Michelis A (2007) Analysis of shrinkage phenomenon of whole sweet cherry fruits ( / Prunus avium) during convective dehydration with very simple models. J Food Eng 79(2):657鈥?61 CrossRef
  34. Ghasemzadeh A, Jaafar HZ, Karimi E (2012) Involvement of salicylic acid on antioxidant and anticancer properties, anthocyanin production and chalcone synthase activity in ginger (Zingiber officinale Roscoe) varieties. Int J Mol Sci 13(11):14828鈥?4844 CrossRef
  35. Nicholson RL, Hammerschmidt R (1992) Phenolic compounds and their role in disease resistance. Annu Rev Phytopathol 30(1):369鈥?89 CrossRef
  36. Janda T, Szalai G, Tari I, Paldi E (1999) Hydroponic treatment with salicylic acid decreases the effects of chilling injury in maize ( / Zea mays L.) plants. Planta 208(2):175鈥?80 CrossRef
  37. Horv谩th E, Szalai G, Janda T (2007) Induction of abiotic stress tolerance by salicylic acid signaling. J Plant Growth Regul 26(3):290鈥?00 CrossRef
  
• 作者单位：Chunfeng Guan (1)
  Xinyu Song (2)
  Jing Ji (1)
  Xiaozhou Li (3)
  Chao Jin (1)
  Wenzhu Guan (2)
  Jing Li (1)
  Gang Wang (1)
  
  1. School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, People鈥檚 Republic of China
  2. School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People鈥檚 Republic of China
  3. Department of Medical Genetics, Tianjin Medical University General Hospital, Tianjin, 300072, People鈥檚 Republic of China
  
• ISSN：1438-2385

文摘

Chalcone isomerase (CHI) is a key gene involved in flavonoid biosynthesis, and flavonoids are important active ingredients in goji berries. Goji berry is a fruit with multi-nutrients consumed all over the world. Up to now, expression patterns of CHI gene and the regulation of flavonoid accumulation in goji berries still remain unknown. In this work, a CHI gene was cloned from fruits of Lycium chinense and was given the name LcCHI. Its relative expression and accumulation of total corresponding flavonoids during fruit maturation were investigated. During the fruit maturation, the expression of LcCHI gene increased gradually, and the accumulation of total flavonoids showed an overall upward trend. This study also showed that the expression of LcCHI gene and the content of total flavonoids in the goji berries could be up-regulated by exogenous salicylic acid (SA) treatment. A reactive oxygen species-dependent signaling pathway was suggested to be involved in this process. This research showed that the expression of LcCHI gene was positively correlated with total flavonoid accumulation and the regulation of them by SA could be a potential approach to produce more desired and valuable flavonoids in goji berries.