Fungicidal and Cytotoxic Activity of a Capsicum chinense Defensin Expressed by Endothelial Cells

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• 作者：Jos¨¦ L. Anaya-L¨®pez ; Joel E. L¨®pez-Meza ; Victor M. Baizabal-Aguirre ; Horacio Cano-Camacho and Alejandra Ochoa-Zarzosa
• 关键词：Antimicrobial peptides ; Candida albicans ; Capsicum chinense ; Cytotoxic ; Defensin ; Endothelial cells
• 刊名：Biotechnology Letters
• 出版年：2006
• 出版时间：July 2006
• 年：2006
• 卷：28
• 期：14
• 页码：1101-1108
• 全文大小：270 KB

文摘

Plant defensins are antimicrobial peptides that exhibit mainly antifungal activity against a broad range of plant fungal pathogens. However, their actions against Candida albicans have not been extensively studied. The mRNA for γ-thionin, a defensin from Capsicum chinense, has been expressed in bovine endothelial cells. The conditioned medium of these cells showed antifungal activity on germ tube formation (60–70 % of inhibition) and on the viability of C. albicans (70–80 % of inhibition). Additionally, C. albicans was not able to penetrate transfected cells. Conditioned medium from these cells also inhibited the viability (80 % ) of the human tumor cell line, HeLa.