Comparison of the genexpert enterovirus assay (GXEA) with real-time one step RT-PCR for the detection of enteroviral RNA in the cerebrospinal fluid of patients with meningitis

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• 作者：JiYoung Hong (1) (3)
  Ahyoun Kim (1)
  Seoyeon Hwang (1)
  Doo-Sung Cheon (1)
  Jong-Hyen Kim (2)
  June-Woo Lee (1)
  Jae-Hak Park (3)
  Byunghak Kang (1)
  
  1. Division of Vaccine Research ; Center for Infectious Diseases ; National Institute of Health ; Korea Center for Disease Control and Prevention ; Cheongju ; Republic of Korea
  3. Department of Laboratory Animal Medicine ; College of Veterinary Medicine ; Seoul National University ; Seoul ; Republic of Korea
  2. Department of Pediatrics ; Catholic University College of Medicine ; Suwon ; Republic of Korea
  
• 关键词：GeneXpert Enterovirus Assay ; Real ; time one step reverse transcription polymerase chain reaction ; Enteroviruses ; Cerebrospinal fluid
• 刊名：Virology Journal
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：12
• 期：1
• 全文大小：386 KB
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• 刊物主题：Virology;
• 出版者：BioMed Central
• ISSN：1743-422X

文摘

Background Enteroviruses (EVs) are the leading cause of aseptic meningitis worldwide. Detection of enteroviral RNA in clinical specimens has been demonstrated to improve the management of patient care, especially that of neonates and young children. Findings To establish a sensitive and reliable assay for routine laboratory diagnosis, we compared the sensitivity and specificity of the GeneXpert Enterovirus Assay (GXEA) with that of the reverse transcription polymerase chain reaction (RT-PCR) based assay referred to as real-time one step RT-PCR (RTo-PCR). The sensitivity/specificity produced by GXEA and RTo-PCR were 100%/100% and 65%/100%, respectively. Conclusions Both methods evaluated in this article can be used for detection of enterovirus in clinical specimens and these nucleic acid amplification methods are useful assays for the diagnosis of enteroviral infection.