Paternity analysis using microsatellite markers to identify pollen donors in an olive grove

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• 作者：Sonali Mookerjee (1)
  Jenny Guerin (1)
  Graham Collins (1)
  Chris Ford (1)
  Margaret Sedgley (1) (2)
  
• 刊名：Theoretical and Applied Genetics
• 出版年：2005
• 出版时间：October 2005
• 年：2005
• 卷：111
• 期：6
• 页码：1174-1182
• 全文大小：431KB
• 参考文献：1. Androulakis I-I, Loupassaki M-H (1990) Studies on self-fertility of some olive cultivars in the area of Crete. Acta Hortic 286:159-62
  2. Angiolillo A, Mencuccini M, Baldoni L (1999) Olive genetic diversity assessed using amplified fragment length polymorphisms. Theor Appl Genet 98:411-21 CrossRef
  3. Atteyyeh A-F, Stosser R, Qrunfleh M (2000) Reproductive biology of the olive ( / Olea europaea L.). J Appl Bot 74:255-70
  4. Bini G, Lensi M (1981) Observazioni su alcuni aspetti dell ‘ontogenesi-fiorale ne olivo. Riv Ortoflorofrutticoltura Ital 65:371-80
  5. Brant B, Witherspoon J, Granger A, Collins G (1999) Identification of pollen donors for sweet cherry cultivars ‘Stella-and ‘Summit-by isozyme analysis. Aust J Exp Agr 39:473-77 CrossRef
  6. Chaix G, Gerber S, Razafimaharo V, Vigneron P, Verhaegen D, Hamon S (2003) Gene flow estimation with microsatellites in a Malagasy seed orchard of / Eucalyptus / grandis. Theor Appl Genet 107:705-12
  7. Cipriani G, Marrazo M-T, Marconi R, Cimato A, Testolin R (2002) Microsatellite markers isolated in olive ( / Olea europaea) are suitable for individual fingerprinting and reveal polymorphism within ancient cultivars. Theor Appl Genet 104:223-28 CrossRef
  8. Cuevas J, Polito V-S (1997) Compatibility relationships in ‘Manzanillo-olive. HortScience 32:1056-058
  9. Cuevas J, Rallo L, Rapoport H-F (1994) Initial fruit set at high temperature in olive, / Olea europaea L. J Hortic Sci 69:655-72
  10. de la Rosa R, James C-M, Tobutt K-R (2002) Isolation and characterisation of polymorphic microsatellites in olive ( / Olea europaea L.) and their transferability to other genera in the Oleaceae. Mol Ecol 2:265-67
  11. de la Rosa R, James C-M, Tobutt K-R (2004) Using microsatellites for paternity testing in olive progenies. HortScience 39:351-54
  12. Gerber S, Chabrier P, Kremer A (2003) FAMOZ: a software for parentage analysis using dominant, codominant, and uniparentally inherited markers. Mol Ecol Notes 3:479-81 CrossRef
  13. Gerber S, Mariette S, Strieff R, Bodenes C, Kremer A (2000) Comparison of microsatellites and amplified fragment length polymorphism markers for parentage analysis. Mol Ecol 9:1037-048 CrossRef
  14. Griggs W-H, Hartmann H-T, Bradley M-V, Iwakiri B-T, Whisler J-E (1975) Olive pollination in California. Division of Agricultural Sciences, University of California, USA
  15. Guerin J, Sweeney S, Collins G, Sedgley M (2002) The development of a genetic database to identify olive cultivars. J Am Soc Hortic Sci 127:977-83
  16. Isagi Y, Kanazashi T, Suzuki W, Tanaka H, Abe T (2004) Highly variable pollination patterns in / Magnolia obovata revealed by microsatellite paternity analysis. Int J Plant Sci 165:1047-053 CrossRef
  17. Jamieson A, Taylor St.C-S (1997) Comparison of three probability formulae for parentage exclusion. Anim Genet 28:397-00 CrossRef
  18. Lavee S, Datt Z (1978) The necessity of cross-pollination for fruit set of ‘Manzanillo-olives. J Hortic Sci 53:261-66
  19. Lavee S, Taryan J, Levin J, Haskal A (2002) The significance of cross-pollination for various olive cultivars under irrigated intensive growing conditions. Olivae 91:25-6
  20. Lewis D (1994) Gametophytic-sporophytic incompatibility. In: Williams E-G, Clarke A-e, Knowx R-B (eds) Genetic control of self-incompatibility and reproductive development in flowering plants. Kluwer, Dordrecht, pp 88-01
  21. Meagher T-R, Thompson E (1986) The relationship between single parent and parent pair genetic likelihoods in genealogy reconstruction. Theor Popul Biol 29:87-06 CrossRef
  22. Mekuria G-T, Collins G-G, Sedgley M (1999) Genetic variability between different accessions of some commercial olive cultivars. J Hortic Sci Biotech 74:309-14
  23. Moutier N, Garcia G, Féral S, Salls J-C (2001) Pollination control in olive orchards. Oliv? 86:35-7
  24. Pinney K, Polito V-S (1990) Olive pollen storage and in?vitro germination. Acta Hortic 286:207-10
  25. Rallo L, Cuevas J, Rapoport H (1990) Fruit set pattern in self and open pollinated olive cultivars. Acta Hortic 286:219-22
  26. Rallo P, Dorado G, Martin A (2000) Development of simple sequence repeats (SSRs) in olive tree ( / Olea europaea L.) Theor Appl Genet 101:984-89 CrossRef
  27. Robeldo-Arnuncio J-J, Gil L (2005) Patterns of pollen dispersal in a small population of / Pinus sylvestris L. revealed by total-exclusion paternity analysis. Heredity 94:13-2 CrossRef
  28. Sedgley M (1994) Self-compatibility in woody horticultural species. In: Williams E-G, Clarke A, Knowx R (eds) Genetic control of self-incompatibility and reproductive development in flowering plants. Kluwer, Dordrecht, pp 141-63
  29. Sefc K-M, Lopes M-S, Mendonca D, Rodriguez dos Santos M, Laimer da Camara Machado M, da Camara Machado A (2000) Identification of microsatellite loci in olive ( / Olea europaea) and their characterisation in Italian and Iberian olive trees. Mol Ecol 9:1171-193 CrossRef
  30. Sibbett G-S, Freeman M, Ferguson L, Polito V-S (1992) Effect of topically applied Sevillano pollen on normal-seeded and parthenocarpic ‘shotberry-fruit set of ‘Manzanillo-olive. HortTechnology 2:228-30
  31. Singh R-P, Kar P-L (1980) Compatibility studies in some olive cultivars. Prog Hortic 12:9-5
  32. Sulaiman Z, Collins G, Witherspoon J, Sedgley M (2004) Identification of pollen donors for the avocado cultivar Gwen in a mixed orchard by isozyme analysis. J Hortic Sci Biotech 79:571-75
  33. Thompson E-A, Meagher T-R (1987) Parental and sib-likelihood in geneology reconstruction. Biometrics 43:585-00
  34. Waits L-P, Luikart G, Taberlet P (2001) Estimating the probability of identity among genotypes in natural populations: cautions and guidelines. Mol Ecol 10:249-56 CrossRef
  35. Wu S, Collins G, Sedgley M (2002) Sexual compatibility within and between olive cultivars. J Hortic Sci Biotech 77:665-73
  
• 作者单位：Sonali Mookerjee (1)
  Jenny Guerin (1)
  Graham Collins (1)
  Chris Ford (1)
  Margaret Sedgley (1) (2)
  
  1. Discipline of Wine and Horticulture, School of Agriculture and Wine, University of Adelaide, Waite Campus, PMB1, Glen Osmond, SA, 5064, Australia
  2. Faculty of The Sciences, University of New England, Armidale, NSW, 2351, Australia
  
• ISSN：1432-2242

文摘

Olive (Olea europaea L.) is a wind-pollinated, allogamous species that is generally not considered to be self-compatible. In addition, cross-incompatibilities exist between cultivars that can result in low fruit set if compatible pollinisers are not planted nearby. In this study, microsatellite markers were used to identify 17 genotypes that were potential pollen donors in a commercial olive orchard. DNA typing with the same primers was also applied to 800 olive embryos collected from five cultivars in the grove over 2?years of study. Pollen donors for the cultivars Barnea, Corregiola, Kalamata, Koroneiki, and Mission were estimated by paternity analysis, based on the parental contribution of alleles in the genotypes of the embryos. The exclusion probability for the marker set was 0.998 and paternity was assigned on the basis of the ‘most likely method- Different pollen donors were identified for each of the maternal cultivars indicating that cross-compatibilities and incompatibilities varied between the genotypes studied. Cross-pollination was the principal method of fertilization, as selfing was only observed in two of the embryos studied and both of these were from the cultivar Mission. This is the first report where these techniques have been applied to survey the pollination patterns in an olive grove. The results indicate that careful planning in orchard design is required for efficient pollination between olive cultivars.