Characterization and Functional Analysis of Class I and II Acidic Chitinase cDNA from Rough Lemon

详细信息    查看全文

• 作者：Kenji GOMI ; Namiko ITOH ; Hiroyuki YAMAMOTO and Kazuya AKIMITSU
• 关键词：Key words ; chitinase ; citrus ; Alternaria alternata ; chitin ; rough lemon ; host ; selective toxin.
• 刊名：Journal of General Plant Pathology
• 出版年：2002
• 出版时间：August 2002
• 年：2002
• 卷：68
• 期：3
• 页码：191-199
• 全文大小：1,205 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Pathology
  Agriculture
  Microbiology
  
• 出版者：Springer Japan
• ISSN：1610-739X

文摘

Two cDNAs encoding acidic chitinase (RlemAchiI and RlemAchiII) were identified from rough lemon (Citrus jambhiri Lush.). The deduced amino acid sequence of RlemAchiI has a typical N-terminal cysteine-rich domain, a proline/ glycine-rich hinge domain and a main structure, indicating that RlemAchiI encodes a class I chitinase. RlemAchiII encodes a class II acidic chitinase. RlemAchiI and RlemAchiII were expressed in Escherichia coli cells, and the fusion proteins had chitinase activity. Northern blot analysis revealed that the corresponding mRNA is inducible by wounding or microbe attack. The transcript was not constitutive but accumulated in leaves within 2 hr after wounding or inoculation with nonpathogenic isolates of Alternaria alternata. The RlemAchi was also induced, but appearance of the signal was delayed after inoculation with the rough lemon pathotype of A. alternata, which is pathogenic to rough lemon and produces a host-selective ACR-toxin.