HERV-K(HML-2) rec and np9 transcripts not restricted to disease but present in many normal human tissues

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• 作者：Katja Schmitt (1) (4)
  Kristina Heyne (2)
  Klaus Roemer (2)
  Eckart Meese (1)
  Jens Mayer (1) (3)
  
  1. Institute of Human Genetics ; Center of Human and Molecular Biology ; Medical Faculty ; University of Saarland ; 66424 ; Homburg/Saar ; Germany
  4. Sanofi-Aventis Deutschland GmbH ; Industriepark Hoechst ; K703 ; Elisabeth Kuhn Street ; Frankfurt/Main ; 65926 ; Germany
  2. Jos茅 Carreras Research Center ; Medical Faculty ; University of Saarland ; 66424 ; Homburg/Saar ; Germany
  3. Center of Human and Molecular Biology ; University of Saarland ; 66424 ; Homburg/Saar ; Germany
  
• 关键词：Human endogenous retrovirus ; Provirus ; Transcription ; Splicing ; HERV ; K Rec protein ; HERV ; K聽Np9 protein ; Retrotransposition ; L1 element
• 刊名：Mobile DNA
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：6
• 期：1
• 全文大小：1,718 KB
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• 刊物主题：Human Genetics; Animal Genetics and Genomics; Plant Genetics & Genomics; Developmental Biology;
• 出版者：BioMed Central
• ISSN：1759-8753

文摘

Background Human endogenous retroviruses of the HERV-K(HML-2) group have been associated with the development of tumor diseases. Various HERV-K(HML-2) loci encode retrovirus-like proteins, and expression of such proteins is upregulated in certain tumor types. HERV-K(HML-2)-encoded Rec and Np9 proteins interact with functionally important cellular proteins and may contribute to tumor development. Though, the biological role of HERV-K(HML-2) transcription and encoded proteins in health and disease is less understood. We therefore investigated transcription specifically of HERV-K(HML-2) rec and np9 mRNAs in a panel of normal human tissues. Results We obtained evidence for rec and np9 mRNA being present in all examined 16 normal tissue types. A total of 18 different HERV-K(HML-2) loci were identified as generating rec or np9 mRNA, among them loci not present in the human reference genome and several of the loci harboring open reading frames for Rec or Np9 proteins. Our analysis identified additional alternative splicing events of HERV-K(HML-2) transcripts, some of them encoding variant Rec/Np9 proteins. We also identified a second HERV-K(HML-2) locus formed by L1-mediated retrotransposition that is likewise transcribed in various human tissues. Conclusions HERV-K(HML-2) rec and np9 transcripts from different HERV-K(HML-2) loci appear to be present in various normal human tissues. It is conceivable that Rec and Np9 proteins and variants of those proteins are part of the proteome of normal human tissues and thus various cell types. Transcription of HERV-K(HML-2) may thus also have functional relevance in normal human cell physiology.