The use of a metal container for vitrification of mouse ovaries, as a clinical grade model for human ovarian tissue cryopreservation, after different times and temperatures of transport

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• 作者：Adriana Bos-Mikich (1)
  Lis Marques (2)
  José Luiz Rodrigues (2)
  Nívia Lothhammer (1)
  Nilo Frantz (3)
  
• 关键词：Metal container ; Vitrification ; Ovaries
• 刊名：Journal of Assisted Reproduction and Genetics
• 出版年：2012
• 出版时间：November 2012
• 年：2012
• 卷：29
• 期：11
• 页码：1267-1271
• 全文大小：266KB
• 参考文献：1. Almodin CG, Minguetti-Camara VC, Meister H, Ferreira JO, Franco RL, Cavalcante AA, Radaelli MR, Bahls AS, Moron AF, Murta CG. Recovery of fertility after grafting of cryopreserved germinative tissue in female rabbits following radiotherapy. Hum Reprod. 2004;19:1287-3. CrossRef
  2. Almodin CG, Minguetti-Camara VC, Meister H, Ceschin AP, Kriger E, Ferreira JO. Recovery of natural fertility after grafting of cryopreserved germinative tissue in ewes subjected to radiotherapy. Fertil Steril. 2004;81:160-. CrossRef
  3. Andersen CY, Rosendahl M, Byskov AG, Loft A, Ottosen C, Dueholm M, Schmidt KL, Andersen AN, Ernst E. Two successful pregnancies following autotransplantation of frozen/thawed ovarian tissue. Hum Reprod. 2008;23:2266-2. CrossRef
  4. Chen SU, Chien CL, Wu MY, Chen TH, Lai SM, Lin CW, et al. Novel direct cover vitrification for cryopreservation of ovarian tissues increases follicle viability and pregnancy capability in mice. Hum Reprod. 2006;21:2794-00. CrossRef
  5. Choi JY, Lee J-Y, Lee EY, Yoon B-K, Boe DS, Choi DS. Cryopreservation of the mouse ovary inhibits the onset of primordial follicle development. Cryobiology. 2007;54:55-2. CrossRef
  6. Donnez J, Dolmans MM, Demylle D, Jadoul P, Pirard C, Squifflet J, Martinez-Madrid B, van Langendonckt A. Livebirth after orthotopic transplantation of cryopreserved ovarian tissue. Lancet. 2004;364:1405-0. CrossRef
  7. Ferreira M, Bos-Mikich A, Frantz N, Rodrigues JL, Brunetto AL, Schwartsmann G. The effects of sample size on the outcome of ovarian tissue cryopreservation. Reprod Dom Anim. 2009;45:99-02. CrossRef
  8. Gandolfi F, Paffoni A, Papasso Brambilla E, Bonetti S, Brevini TA, Ragni G. Efficiency of equilibrium cooling and vitrification procedures for the cryopreservation of ovarian tissue: comparative analysis between human and animal models. Fertil Steril. 2006;85:1150-. CrossRef
  9. Henry L, Fransolet M, Labied S, Blacher S, Munaut C, Colige A, Kirschvink N, No?l A, Nisolle M, Foidart J-M. Does isoforms 111 and 165 of vascular endothelial growth factor (VEGF111 and 165) improve transplanted ovarian tissue survival? J Assist Reprod Genet. 2011;28:1007-4. CrossRef
  10. Isachenko V, Lapidus I, Isachenko E, Krivokharchenko A, Kreienberg R, Woriedh M, Bader M, Weiss JM. Human ovarian tissue vitrification versus conventional freezing: morphological, endocrinological, and molecular biological evaluation. Reproduction. 2009;138:319-7. CrossRef
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  12. Jin SY, Lei L, Shea LD, Zelinski MB, Stouffer RL, Woodruff TK. Markers of growth and development in primate primordial follicles are preserved after slow cryopreservation. Fertil Steril. 2010;93:2627-2. CrossRef
  13. Keros V, Xella S, Hultenby K, Pettersson K, Sheikhi M, Volpe A, Hreinsson J, Hovatta O. Vitrification versus controlled-rate freezing in cryopreservation of human ovarian tissue. Hum Reprod. 2009;24:1670-3. CrossRef
  14. Labied S, Delforges Y, Blacher S, Munaut C, Signole N, Colige A, Janssen L, Henry L, d’Hauterive SP, Jouan C, Kirschvink N, No?l A, Nisolle M, Foidart J-M. Isoform 111 of vascular endothelial growth factor (VEGF111) improves angiogenesis of ovarian tissue xenotransplantation. J Assist Reprod Genet. 2011;28:1007-4. CrossRef
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  17. Sheikhi M, Hultenby K, Niklasson B, Lundqvist M, Hovatta O. Clinical grade vitrification of human ovarian tissue: an ultrastructural analysis of follicles and stroma in vitrified tissue. Hum Reprod. 2011;26:594-03. CrossRef
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• 作者单位：Adriana Bos-Mikich (1)
  Lis Marques (2)
  José Luiz Rodrigues (2)
  Nívia Lothhammer (1)
  Nilo Frantz (3)
  
  1. Department of Morphological Sciences, ICBS, Federal University of Rio Grande do Sul, CEP: 90.050-170, Porto Alegre, RS, Brazil
  2. Reproduction Biotechnologies Laboratory, Veterinay School, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil
  3. Nilo Frantz Research and Human Reproduction Centre, CEP: 90.480-003, Porto Alegre, RS, Brazil
  
• ISSN：1573-7330

文摘

Purpose Cryopreservation of ovarian tissue is paramount for fertility preservation, with important clinical applications, especially for women suffering from an oncological condition. Several cryopreservation methodologies have been tried in search of better outcomes, especially in terms of primor-dial and primary follicles integrity post-cryopreservation. Vitrification has successfully been applied to ovarian tissue using different carriers for tissue exposure to the liquid nitrogen (LN2). Methods We developed an enclosed metal vessel, which has the advantage of a faster heat transfer, when in contact with LN2 avoiding at the same time, the direct contact with tissue. Additionally, we assessed the effect of different times and temperatures of transport between the collection of mouse ovaries and the beginning of cryopreservation, on follicular morphology after vitrification. Results Our results suggest that 37?°C and R.T. help to maintain normal primordial and primary follicle morphology for up to 4?hrs after collection and beginning of vitrification in a metal container. Conclusion These data show that the metal container is an appropriate carrier for mouse ovary vitrification. The rate of morphologically normal primordial follicles up to 4?hrs.