Synthesis of silver nanoparticles modified with lysozyme and sodium dioctyl sulfosuccinate and comparison of their biological activities

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• 作者：O. Yu. Golubeva ; V. V. Golubkov ; V. A. Yukhnev…
• 关键词：silver nanoparticles ; lysozyme ; AOT ; antimicrobial activity ; antibiotics
• 刊名：Glass Physics and Chemistry
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：43
• 期：1
• 页码：63-69
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Ceramics, Glass, Composites, Natural Materials; Characterization and Evaluation of Materials; Materials Science, general; Physical Chemistry;
• 出版者：Pleiades Publishing
• ISSN：1608-313X
• 卷排序：43

文摘

Silver nanoparticles have been fabricated by the method of chemical reduction in solution using different types of stabilizers: an organic low-molecular compound—sodium dioctyl sulfosuccinate (AOT)— and a natural biologically active substance—antimicrobial cationic polypeptide lysozyme. According to studies of the produced hydrosols, the average size of the shell-coated particles is 20–25 nm. The biological activity of the obtained bioconjugates toward Gram-negative (Escherichia coli ML35p, Pseudomonas aeruginosa (clinical isolate)) and Gram-positive (Listeria monocytogenes EGD (АТСС ВАА-679), MRSA ATCC 33591 (Staphylococcus aureus resistant to methicillin)) bacteria has been investigated by the methods of radial diffusion in an agarose gel and serial dilution in a liquid nutritional medium. It has been established that the antimicrobial activity of the bioconjugates depends on the nature of the used stabilizer.