Engineering Biological Approaches for Detection of Toxic Compounds: A New Microbial Biosensor Based on the Pseudomonas putida TtgR Repressor

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• 作者：Manuel Espinosa-Urgel ; Luis Serrano ; Juan Luis Ramos…
• 关键词：Antimicrobial resistance ; Microbial biosensor ; Pseudomonas ; Extrusion efflux pump ; TtgR repressor
• 刊名：Molecular Biotechnology
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：57
• 期：6
• 页码：558-564
• 全文大小：908 KB
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• 作者单位：Manuel Espinosa-Urgel (1)
  Luis Serrano (2)
  Juan Luis Ramos (1)
  Ana María Fernández-Escamilla (1)
  
  1. Department of Environmental Protection, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), C/Profesor Albareda, 1, 18008, Granada, Spain
  2. Systems Biology Research Unit, European Molecular Biology Laboratory (EMBL), Centre for Genomic Regulation, C/Dr. Aiguader, 88, 08003, Barcelona, Spain
  
• 刊物主题：Biotechnology; Biochemistry, general; Cell Biology; Protein Science; Biological Techniques; Human Genetics;
• 出版者：Springer US
• ISSN：1559-0305

文摘

Environmental contamination by toxic organic compounds and antimicrobials is one of the causes for the recent surge of multidrug-resistant pathogenic bacteria. Monitoring contamination is therefore the first step in containment of antimicrobial resistance and requires the development of simple, sensitive, and quantitative tools that detect a broad spectrum of toxic compounds. In this study, we have engineered a new microbial biosensor based on the ttgR-regulated promoter that controls expression of the TtgABC extrusion efflux pump of Pseudomonas putida, coupled to a gfp reporter. The system was introduced in P.?putida DOT-T1E, a strain characterized by its ability to survive in the presence of high concentrations of diverse toxic organic compounds. This whole-cell biosensor is capable to detect a wide range of structurally diverse antibiotics, as well as compounds such as toluene or flavonoids.