MxA transcripts with distinct first exons and modulation of gene expression levels by single-nucleotide polymorphisms in human bronchial epithelial cells

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• 作者：Satoshi Noguchi (1) (2)
  Minako Hijikata (1)
  Emi Hamano (1) (2)
  Ikumi Matsushita (1)
  Hideyuki Ito (3)
  Jun Ohashi (4)
  Takahide Nagase (2)
  Naoto Keicho (1)
  
• 关键词：Myxovirus resistance A ; Single ; nucleotide polymorphism ; Human bronchial epithelial cells ; Transcript variants
• 刊名：Immunogenetics
• 出版年：2013
• 出版时间：February 2013
• 年：2013
• 卷：65
• 期：2
• 页码：107-114
• 全文大小：446KB
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• 作者单位：Satoshi Noguchi (1) (2)
  Minako Hijikata (1)
  Emi Hamano (1) (2)
  Ikumi Matsushita (1)
  Hideyuki Ito (3)
  Jun Ohashi (4)
  Takahide Nagase (2)
  Naoto Keicho (1)
  
  1. Department of Respiratory Diseases, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku-ku, Tokyo, 162-8655, Japan
  2. Department of Respiratory Medicine, University of Tokyo Hospital, Tokyo, 113-0033, Japan
  3. Department of Thoracic Surgery, National Center for Global Health and Medicine, Tokyo, 162-8655, Japan
  4. Molecular and Genetic Epidemiology, Faculty of Medicine, University of Tsukuba, Ibaraki, 305-8575, Japan
  
• ISSN：1432-1211

文摘

Myxovirus resistance A (MxA) is a major interferon (IFN)-inducible antiviral protein. Promoter single-nucleotide polymorphisms (SNPs) of MxA near the IFN-stimulated response element (ISRE) have been frequently associated with various viral diseases, including emerging respiratory infections. We investigated the expression profile of MxA transcripts with distinct first exons in human bronchial epithelial cells. For primary culture, the bronchial epithelium was isolated from lung tissues with different genotypes, and total RNA was subjected to real-time reverse transcription polymerase chain reaction. The previously reported MxA transcript (T1) and a recently registered transcript with a distinct 5-first exon (T0) were identified. IFN-β and polyinosinic–polycytidylic acid induced approximately 100-fold higher expression of the T1 transcript than that of the T0 transcript, which also had a potential ISRE motif near its transcription start site. Even without inducers, the T1 transcript accounted for approximately two thirds of the total expression of MxA, levels of which were significantly associated with its promoter and exon 1 SNPs (rs17000900, rs2071430, and rs464138). Our results suggest that MxA observed in respiratory viral infections is possibly dominated by the T1 transcript and partly influenced by relevant 5-SNPs.