Nucleoprotein-based indirect enzyme-linked immunosorbent assay (indirect ELISA) for detecting antibodies specific to Ebola virus and Marbug virus

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• 作者：Yi Huang ; Youjie Zhu ; Mengshi Yang ; Zhenqing Zhang ; Donglin Song…
• 关键词：Ebola virus ; Marburg virus ; recombinant nucleoproteins ; indirect ELISA
• 刊名：Virologica Sinica
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：29
• 期：6
• 页码：372-380
• 全文大小：855 KB
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• 作者单位：Yi Huang (1)
  Youjie Zhu (1) (2)
  Mengshi Yang (1)
  Zhenqing Zhang (1)
  Donglin Song (1)
  Zhiming Yuan (1)
  
  1. Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China
  2. University of the Chinese Academy of Sciences, Beijing, 100039, China
  
• 刊物主题：Virology; Medical Microbiology; Oncology; Biochemistry, general; Microbiology; Microbial Genetics and Genomics;
• 出版者：Springer Berlin Heidelberg
• ISSN：1995-820X

文摘

Full-length nucleoproteins from Ebola and Marburg viruses were expressed as His-tagged recombinant proteins in Escherichia coli and nucleoprotein-based enzyme-linked immunosorbent assays (ELISAs) were established for the detection of antibodies specific to Ebola and Marburg viruses. The ELISAs were evaluated by testing antisera collected from rabbit immunized with Ebola and Marburg virus nucleoproteins. Although little cross-reactivity of antibodies was observed in anti-Ebola virus nucleoprotein rabbit antisera, the highest reactions to immunoglobulin G (IgG) were uniformly detected against the nucleoprotein antigens of homologous viruses. We further evaluated the ELISA’s ability to detect antibodies to Ebola and Marburg viruses using human sera samples collected from individuals passing through the Guangdong port of entry. With a threshold set at the mean plus three standard deviations of average optical densities of sera tested, the ELISA systems using these two recombinant nucleoproteins have good sensitivity and specificity. These results demonstrate the usefulness of ELISA for diagnostics as well as ecological and serosurvey studies of Ebola and Marburg virus infection.