Fibroblast Migration in 3D is Controlled by Haptotaxis in a Non-muscle Myosin II-Dependent Manner

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• 作者：O. Moreno-Arotzena ; C. Borau ; N. Movilla…
• 关键词：Microfluidics ; Hydrogel ; Collagen ; Fibrin ; Chemotaxis ; Mechanical properties
• 刊名：Annals of Biomedical Engineering
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：43
• 期：12
• 页码：3025-3039
• 全文大小：2,889 KB
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• 作者单位：O. Moreno-Arotzena (1)
  C. Borau (1)
  N. Movilla (1)
  M. Vicente-Manzanares (2)
  J. M. Garc铆a-Aznar (1)
  
  1. Multiscale in Mechanical and Biological Engineering (M2BE), Department of Mechanical Engineering, Aragon Institute of Engineering Research (I3A), University of Zaragoza, 50018, Saragossa, Spain
  2. Department of Medicine, Hospital Universitario de la Princesa, Universidad Autonoma de Madrid School of Medicine, 28006, Madrid, Spain
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Biomedicine
  Biomedical Engineering
  Biophysics and Biomedical Physics
  Mechanics
  Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-9686

文摘

Cell migration in 3D is a key process in many physiological and pathological processes. Although valuable knowledge has been accumulated through analysis of various 2D models, some of these insights are not directly applicable to migration in 3D. In this study, we have confined biomimetic hydrogels within microfluidic platforms in the presence of a chemoattractant (platelet-derived growth factor-BB). We have characterized the migratory responses of human fibroblasts within them, particularly focusing on the role of non-muscle myosin II. Our results indicate a prominent role for myosin II in the integration of chemotactic and haptotactic migratory responses of fibroblasts in 3D confined environments. Keywords Microfluidics Hydrogel Collagen Fibrin Chemotaxis Mechanical properties