The Oncofetal Gene Survivin Promotes Cell Proliferation and Survival in Primary Human Osteoblastic Cells

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• 作者：1. Department of Orthopedic Surgery ; University of Regensburg ; Asklepios Klinikum Bad Abbach ; Kaiser Karl V Allee 3 ; Bad Abbach ; 93077 Germany2. Renal Section ; Division of Immunology and Inflammation (Medicine) ; Imperial College London ; Hammersmith Hospital ; London ; UK3. Department of Nephrology and Hypertension ; University of Erlangen-Nuremberg ; Erlangen ; Germany4. Department of Trauma and Plastic Surgery ; University of Regensburg ; Regensburg ; Germany
• 关键词：Osteoblast 211 ; Apoptosis 211 ; Proliferation 211 ; Survivin
• 刊名：Calcified Tissue International
• 出版年：2011
• 出版时间：September 2011
• 年：2011
• 卷：89
• 期：3
• 页码：211-220
• 全文大小：526.5 KB
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• 作者单位：http://www.springerlink.com/content/t4647x65j523081x/
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Endocrinology
  Orthopedics
  Cell Biology
  
• 出版者：Springer New York
• ISSN：1432-0827

文摘

Survivin, the smallest member of the inhibitor of apoptosis gene family, is critical for the regulation of mitosis and maintenance of cell viability during embryonic development and cancer, while not being detectable in most adult differentiated tissues. We know little about whether survivin plays any physiological or pathophysiological role in the adult musculoskeletal system. We studied the expression of survivin in primary human osteoblastic cells and its biological functions in vitro. Survivin was detected by immunoblotting and real-time PCR. Subcellular localization was analyzed by immunofluorescence. Transfection of siRNA and plasmids coding for wild-type survivin was performed to study survivin function, i.e., proliferation and apoptosis assays. Survivin mRNA and protein are expressed in primary human osteoblastic cells. During interphase survivin localizes predominantly to the cytoplasmic compartment, which is relevant for the organization of the spindle apparatus during mitosis. Survivin knockdown resulted in an arrest of the cell cycle at the G₂/M phase and increased rates of apoptosis. Elevated levels of survivin in primary human osteoblasts enhanced proliferation and cell viability. Taken together, we demonstrate for the first time that survivin is expressed in primary human osteoblastic cells on the mRNA and protein levels. Our results indicate that survivin is a critical factor for cell division and cell viability in primary human osteoblastic cells. Learning more about survivin217;s role in human osteoblasts could be an important step toward understanding the complex processes involved in bone homeostasis and remodeling.