Regulation of podocalyxin expression in the kidney of streptozotocin-induced diabetic rats with Chinese herbs (Yishen capsule)

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• 作者：Jingai Fang (1)
  Hongkun Wei (1)
  Yanyan Sun (1)
  Xiaodong Zhang (1)
  Wenyuan Liu (1)
  Qintao Chang (1)
  Ruihua Wang (1)
  Yuewen Gong (2)
  
• 刊名：BMC Complementary and Alternative Medicine
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：13
• 期：1
• 全文大小：817KB
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  38. The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6882/13/76/prepub
  
• 作者单位：Jingai Fang (1)
  Hongkun Wei (1)
  Yanyan Sun (1)
  Xiaodong Zhang (1)
  Wenyuan Liu (1)
  Qintao Chang (1)
  Ruihua Wang (1)
  Yuewen Gong (2)
  
  1. Department of Nephrology, The First Affiliated Hospital to the Shanxi Medical University, 56 Xinjian South Road, Taiyuan, Shanxi Province, 030001, People鈥檚 Republic of China
  2. Faculty of Pharmacy, University of Manitoba, Winnipeg, R3E 0 T5, Canada
  

文摘

Background Diabetic nephropathy is an emergent issue in China with increase in patients with type II diabetes. There are several successful Chinese herbal products for the treatment of patients with diabetic nephropathy in China. However, the mechanisms mediating the biological activity of these products are still unclear. Podocalyxin is a sialoprotein critical to maintaining integrity of filtration function of glomerulus. Methods By employing streptozotocin-induced diabetic rats and a Chinese herb formulation (Yishen capsule), we examined the regulation of podocalyxin expression in the kidney by Yishen capsule through immunofluorescent staining and reverse transcriptase polymerase chain reaction. Results After injection of STZ, there were significant increase in both blood glucose and urinary protein. Serum creatinine and BUN were also increased in rats with injection of STZ. Moreover, expression of podocalyxin in the glomerulus was gradually reduced after injection of STZ. There was also a loss of podocyte foot processes in the glomerular basement membrane. However, Yishen capsule or benazepril was able to restore the expression of podocalyxin and podocyte foot processes in the kidney. Although Yishen capsule could reduce urinary protein level, it has little effect on blood glucose level in the rats injected with STZ. Conclusions Yishen capsule could attenuate the loss of podocalyxin in the glomerulus of rats injected with STZ.