Temporal Variations in Diazotrophic Communities and nifH Transcripts Level Across the Agricultural and Fallow Land at Jaipur, Rajasthan, India

详细信息    查看全文

• 作者：Sushma Sharma ; Dileep K. Singh
• 关键词：Biological nitrogen fixation ; Diazotrophic ; Temporal ; nifH ; Denaturing gradient gel electrophoresis ; Real time polymerase chain reaction
• 刊名：Indian Journal of Microbiology
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：57
• 期：1
• 页码：92-99
• 全文大小：
• 刊物主题：Microbiology; Medical Microbiology;
• 出版者：Springer India
• ISSN：0973-7715
• 卷排序：57

文摘

Biological nitrogen fixation is one of the most important nutrient processes in the ecosystem. Many studies have estimated the variations in soil diazotrophic communities across the globe. To understand the dynamics of nitrogen fixing bacterial communities and their response to the environmental changes, it is important to study the temporal variability of these communities. Using DNA fingerprinting method, denaturing gradient gel electrophoresis and real time polymerase chain reaction of the nifH gene, we have found that the agricultural land harbored unique nitrogen fixing communities that revealed different temporal patterns and abundance. Variation in soil moisture, organic carbon content, ammonium and nitrate concentrations may be the main factors which influenced the diazotrophic community composition and nifH gene abundance. Azospirillum species were more dominant in the agricultural soil. Unique environmental factors and agricultural practices were responsible for the temporal shifts in bacterial community structures and nifH transcripts level. Our study expands understanding of the influence of environmental factors on diazotrophic population that contributes to the nitrogen pool.