Effect of homogeneous and heterogeneous supply of nitrate and ammonium on nitrogen uptake and distribution in tomato seedlings

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• 作者：Caixia Dong (1) cxdong@njau.edu.cn
  Yinglin Lu (2) fanyang21st@126.com
  YiYong Zhu (1) yiyong1973@njau.edu.cn
  Yi Zhou (3) zhouyi_nwau@sohu.com
  Yangchun Xu (1) ycxu@njau.edu.cn
  Qirong Shen (1) qirongshen@njau.edu.cn
• 关键词：Tomato (Lycopersicon esculentum Mill.) &#8211 ; NO3 − ; &#8211 ; NH4 + &#8211 ; Nitrogen uptake &#8211 ; Nitrogen distribution &#8211 ; Homogeneous and heterogeneous supply
• 刊名：Plant Growth Regulation
• 出版年：2012
• 出版时间：November 2012
• 年：2012
• 卷：68
• 期：2
• 页码：271-280
• 全文大小：361.2 KB
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• 作者单位：1. College of Resources and Environmental Sciences, Nanjing Agricultural University, Weigang 1, Nanjing, 210095 Jiangsu province, China2. Guangzhou Sugarcane Industry Research Institute, Guangzhou, 510316 China3. Anhui Science and Technology University, Fengyang, 233100 China
• ISSN：1573-5087

文摘

To evaluate the effects of homogeneous or heterogeneous supply of different sources of nitrogen (N) on N uptake and distribution in tomato seedlings, hydroponic experiments with 15N tracers were conducted. Under homogeneous condition, NO₃ − and NH₄ + were supplied as the ratio of 100:0 (100-0NA), 75:25 (75-25 NA), and 50:50 (50-50NA) for all roots of tomato seedlings. Heterogeneous supply of N (localized N supply) was conducted by a split-root experiment with three treatments: both half of the roots supplied with NO₃ − (N|N, as the control), a half of the roots supplied with NH₄ + (N|A), and a half of the roots supplied with NO₃ − and NH₄ + at a ratio of 75:25 (N|AN). The results showed that (1) homogeneous supply of NO₃ − and NH₄ + at the ratio of 75:25 led to the highest root and shoot biomass and a higher total N uptake. 15NO₃ − absorption was not affected by decreased NO₃ − percentage from 100 to 75 %, and increase of NH₄ + ratio from 25 to 50 % showed no effect on 15NH₄ + absorption. Similar amount of 15NH₄ +-N was absorbed by roots under N|A and N|AN treatments in the localized supply. Spatially separated supply of NO₃ − and NH₄ + led to a more than 3 times higher 15N accumulation in NO₃ −-fed root than NH₄ +-fed root in N|A. Half-roots supplied by mixed NO₃ − and NH₄ + at the ratio of 75:25 in N|AN resulted in higher 15N accumulation in the shoot. (2) In plant tissues, the amount of 15N deriving from NO₃ − in root, stem, and leaves was all decreased by 50 % NH₄ + in the whole-root system. In N|A treatment in the split-root system, however, the amount of leaf 15N deriving from sole NO₃ − was increased by 66 % and that coming from sole NH₄ + decreased by 73 %, in comparison with N|N. The amount of 15N coming from the mixed N source was found to be more than 30 % higher in stem, leaves, and flower. Taken together, our results suggested that whether homogeneous or heterogeneous supply of NO₃ − and NH₄ + significantly influenced the N uptake and distribution in the plant and combined application of NO₃ − and NH₄ + was approved to be superior to plant growth and N uptake.