Venom-gland transcriptome and venom proteome of the Malaysian king cobra (Ophiophagus hannah)

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• 作者：Choo Hock Tan ; Kae Yi Tan ; Shin Yee Fung ; Nget Hong Tan
• 关键词：Ophiophagus hannah ; King cobra ; Venom proteome ; Venom gland transcriptome ; Nano ; LCMS ; ESI ; LCMS/MS
• 刊名：BMC Genomics
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：16
• 期：1
• 全文大小：1,899 KB
• 刊物主题：Life Sciences, general; Microarrays; Proteomics; Animal Genetics and Genomics; Microbial Genetics and Genomics; Plant Genetics & Genomics;
• 出版者：BioMed Central
• ISSN：1471-2164

文摘

Background The king cobra (Ophiophagus hannah) is widely distributed throughout many parts of Asia. This study aims to investigate the complexity of Malaysian Ophiophagus hannah (MOh) venom for a better understanding of king cobra venom variation and its envenoming pathophysiology. The venom gland transcriptome was investigated using the Illumina HiSeq™ platform, while the venom proteome was profiled by 1D-SDS-PAGE-nano-ESI-LCMS/MS.