Touchdown PCR assay for the rapid diagnosis of tawny rust caused by Macruropyxis fulva on sugarcane

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• 作者：Lauren Anne Martin ; RS Rutherford ; SA McFarlane
• 关键词：Tawny rust ; Macruropyxis fulva ; Diagnostic assays ; Specific PCR ; Touchdown PCR
• 刊名：Australasian Plant Pathology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：46
• 期：1
• 页码：103-105
• 全文大小：
• 刊物主题：Plant Pathology; Plant Sciences; Agriculture; Entomology; Ecology;
• 出版者：Springer Netherlands
• ISSN：1448-6032
• 卷排序：46

文摘

Macruropyxis fulva is a newly described rust pathogen causing tawny rust of sugarcane in southern Africa. It has been observed on a number of commercial South African varieties grown under varying environmental conditions. Macruropyxis fulva is the third rust pathogen, together with Puccinia melanocephala (brown rust) and Puccinia kuehnii (orange rust) known to infect this crop. A touchdown PCR assay specific for M. fulva was developed to rapidly and reliably differentiate this new rust from the other two rusts infecting sugarcane.