Penicillin production in industrial strain Penicillium chrysogenum P2niaD18 is not dependent on the copy number of biosynthesis genes

详细信息    查看全文

• 作者：Sandra Ziemons ; Katerina Koutsantas ; Kordula Becker ; Tim Dahlmann…
• 关键词：Penicillium chrysogenum ; Beta ; lactam antibiotics ; Production strain ; Gene cluster amplification
• 刊名：BMC Biotechnology
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：17
• 期：1
• 全文大小：2743KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Applied Microbiology; Biotechnology; Biochemical Engineering; Genetic Engineering; Plant Breeding/Biotechnology; Biomedical Engineering/Biotechnology;
• 出版者：BioMed Central
• ISSN：1472-6750
• 卷排序：17

文摘

BackgroundMulti-copy gene integration into microbial genomes is a conventional tool for obtaining improved gene expression. For Penicillium chrysogenum, the fungal producer of the beta-lactam antibiotic penicillin, many production strains carry multiple copies of the penicillin biosynthesis gene cluster. This discovery led to the generally accepted view that high penicillin titers are the result of multiple copies of penicillin genes. Here we investigated strain P2niaD18, a production line that carries only two copies of the penicillin gene cluster.