Extraction and electrophoretic analysis of large dsRNAs from desiccated plant tissues infected with plant viruses and biotrophic fungi

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• 作者：S. Khankhum ; C. Escalante ; E. Rodrigues de Souto and…
• 关键词：Biotrophic fungi ; Large dsRNAs ; Plant virus ; Powdery mildew ; Rust
• 刊名：European Journal of Plant Pathology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：147
• 期：2
• 页码：431-441
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Plant Pathology; Plant Sciences; Ecology; Agriculture; Life Sciences, general;
• 出版者：Springer Netherlands
• ISSN：1573-8469
• 卷排序：147

文摘

Extraction and electrophoretic analysis of large dsRNAs from plant and fungal tissues has been used successfully to detect RNA viruses infecting plants, fungi, and oomycetes. We modified a previously reported dsRNA extraction protocol and used it to detect a wide variety of plant and putative fungal viruses from infected plant tissues. The modified protocol was used successfully to extract large dsRNAs from 50 to 70 mg of desiccated plant tissues infected with acute and persistent RNA viruses and from plant tissues infected with biotrophic fungi causing rusts and powdery mildew diseases. The protocol proved to be efficient, fast, economic and versatile and requires relatively small amounts of desiccated tissue.