16S rDNA sequence analysis of witches’ broom phytoplasma isolates from Chinese jujube in North China
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  • 作者：Jiaodi Bu ; Long Peng ; Mengjun Liu ; Jin Zhao
  • 关键词：Jujube witches’ broom disease ; Phytoplasma ; 16S rDNA ; North China
  • 刊名：Australasian Plant Pathology
  • 出版年：2016
  • 出版时间：February 2016
  • 年：2016
  • 卷：45
  • 期：1
  • 页码：119-122
  • 全文大小：279 KB
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  • 作者单位：Jiaodi Bu (1)
    Long Peng (1)
    Mengjun Liu (2)
    Jin Zhao (1)

    1. College of Life Science, Agricultural University of Hebei, Baoding, 071000, China
    2. Research Center for Chinese Jujube, Agricultural University of Hebei, Baoding, 071000, China
  • 刊物主题：Plant Pathology; Plant Sciences; Agriculture; Entomology; Ecology;
  • 出版者：Springer Netherlands
  • ISSN：1448-6032
文摘
Phytoplasmas are agriculturally important pathogens for more than one thousand plant species. Jujube witches’ broom (JWB), a plant disease caused by phytoplasma, is highly fatal in Chinese jujube, an important fruit tree and medicinal crop in Asia. The 16S rDNA of 15 JWB isolates from 4 provinces in North China were amplified, cloned and sequenced. The isolates were over 99.8 % homologous, indicating that JWB phytoplasma is highly conserved. Phylogenetic analysis of the 16S rDNA sequences from those isolates, together with the representative phytoplasma sequences from other host species, showed that all JWB isolates belong to a subgroup of 16Sr V group and are closely related to Elm yellows (EY) phytoplasma. Virtual RFLP and single-nucleotide polymorphism (SNP) analysis of 16S rDNA also revealed that JWB isolates have a relatively low level of genetic diversity and that their SNP sites exhibit a strong bias toward A/G variation when compared with other phytoplasmas. The high similarity among JWB isolates from different regions and cultivars is consistent with the conserved symptoms of the disease and the wide infectivity of JWB phytoplasmas. This result provides valuable information to better understand the genetic diversity of JWB phytoplasmas. Keywords Jujube witches’ broom disease Phytoplasma 16S rDNA North China
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