Maturation of Anthurium andraeanum cv. Eidibel somatic embryos from nodal segments

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• 作者：Marcos Vinícius Marques Pinheiro (1)
  Fabrina Bolzan Martins (2)
  Ana Claudia Ferreira da Cruz (1)
  Ana Cristina Portugal Pinto de Carvalho (3)
  Marília Contim Ventrella (1)
  Wagner Campos Otoni (1) (4)
  
• 关键词：AA2 medium ; Kinetin ; Liquid medium ; Pierik medium ; Somatic embryogenesis
• 刊名：In Vitro Cellular & Developmental Biology - Plant
• 出版年：2013
• 出版时间：June 2013
• 年：2013
• 卷：49
• 期：3
• 页码：304-312
• 全文大小：783KB
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• 作者单位：Marcos Vinícius Marques Pinheiro (1)
  Fabrina Bolzan Martins (2)
  Ana Claudia Ferreira da Cruz (1)
  Ana Cristina Portugal Pinto de Carvalho (3)
  Marília Contim Ventrella (1)
  Wagner Campos Otoni (1) (4)
  
  1. Departamento de Biologia Vegetal, Universidade Federal de Vi?osa, 36570-000, Vi?osa, MG, Brazil
  2. Instituto de Recursos Naturais, UNIFEI, Campus Prof. José Rodrigues Seabra, 37500-903, Itajubá, MG, Brazil
  3. Embrapa Agroindústria Tropical, Rua Dra. Sara Mesquita, n° 2270, Bairro Pici, 60511-110, Fortaleza, CE, Brazil
  4. Plant Biology Department, Plant Tissue Culture Laboratory/BIOAGRO, Federal University of Vi?osa, University Campus, P.H. Rolfs Avenue, 36570-000, Vi?osa, MG, Brazil
  

文摘

Maturation of somatic embryos of Anthurium andraeanum cv. Eidibel from embryogenic callus was evaluated. Following induction of embryogenic calli from nodal segments, tissues were transferred to 125-mL Erlenmeyer flasks containing 25?mL liquid medium, with 0, 4.52, or 9.05?μM 2,4-dichlorophenoxyacetic acid and 0, 0.47, or 2.32?μM kinetin. Callus cultures were maintained in a dark growth room at 25?±-°C. At 45?d, the mass of embryogenic calli, number of primary and secondary somatic embryos, and percentage browning were evaluated. Nonparametric tests were used to evaluate color, texture, and somatic embryo development. The highest yield of somatic embryos was in the medium with 0.47?μM kinetin. Calli were friable, with a lower yield of secondary somatic embryos, and have minimal browning. Histology revealed polar globular somatic embryos and mature somatic embryos with defined apical and root meristematic zones, axillary buds, and primary leaves. These are important features for converting somatic embryos into plantlets.