Alr2954 of Anabaena sp. PCC 7120 with ADP-ribose pyrophosphatase activity bestows abiotic stress tolerance in Escherichia coli

详细信息    查看全文

• 作者：Prashant Kumar Singh ; Alok Kumar Shrivastava…
• 关键词：Anabaena sp. PCC 7120 ; Escherichia coli ; ADP ; ribose pyrophosphatase ; Hypothetical protein ; Homology modeling ; Molecular docking ; Cloning and expression
• 刊名：Functional & Integrative Genomics
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：17
• 期：1
• 页码：39-52
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Cell Biology; Plant Genetics & Genomics; Microbial Genetics and Genomics; Biochemistry, general; Bioinformatics; Animal Genetics and Genomics;
• 出版者：Springer Berlin Heidelberg
• ISSN：1438-7948
• 卷排序：17

文摘

In silico derived properties on experimental validation revealed that hypothetical protein Alr2954 of Anabaena sp. PCC7120 is ADP-ribose pyrophosphatase, which belongs to nudix hydrolase superfamily. Presence of ADP-ribose binding site was attested by ADP-ribose pyrophosphatase activity (K_m 44.71 ± 8.043 mM, V_max 7.128 ± 0.417 μmol min−1 mg protein−1, and K_cat/K_m 9.438 × 104 μM−1 min−1). Besides ADP-ribose, the enzyme efficiently hydrolyzed various nucleoside phosphatases such as 8-oxo-dGDP, 8-oxo-dADP, 8-oxo-dGTP, 8-oxo-dATP, GDP-mannose, ADP-glucose, and NADH. qRT-PCR analysis of alr2954 showed significant expression under different abiotic stresses reconfirming its role in stress tolerance. Thus, Alr2954 qualifies to be a member of nudix hydrolase superfamily, which serves as ADP-ribose pyrophosphatase and assists in multiple abiotic stress tolerance.