Characterization of a nucleotide-oligomerization domain (NOD) like receptor C5 (NLRC5) subfamily member from black rockfish (Sebastes schlegelii), portraying its transcriptional responses against immune stimulants

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• 作者：Don Anushka Sandaruwan Elvitigala ; William Shanthakumar Thulasitha…
• 关键词：NLRC5 ; Black rockfish ; Tissue specific mRNA expression ; Immune ; stimulation ; Transcriptional modulation
• 刊名：Genes & Genomics
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：38
• 期：3
• 页码：303-310
• 全文大小：579 KB
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• 作者单位：Don Anushka Sandaruwan Elvitigala (1) (2)
  William Shanthakumar Thulasitha (1) (2)
  Jehee Lee (1) (2)
  
  1. Marine Molecular Genetics Lab, Department of Marine Life Sciences, School of Marine Biomedical Sciences, College of Ocean Science, Jeju National University, 66 Jejudaehakno, Ara-Dong, Jeju, 690-756, Jeju Self-Governing Province, Republic of Korea
  2. Fish Vaccine Research Center, Jeju National University, Jeju, 690-756, Jeju Special Self-Governing Province, Republic of Korea
  
• 刊物主题：Microbial Genetics and Genomics; Plant Genetics & Genomics; Animal Genetics and Genomics; Human Genetics;
• 出版者：Springer Netherlands
• ISSN：2092-9293

文摘

Nucleotide-oligomerization domain like receptors (NLRs) are recently identified group of pattern recognition receptors which involve in sensing broad range of pathogen associated molecular patterns or damage associated molecular patterns to trigger corresponding immune responses in host cells. In this study, we identified and characterized a NLRC5 family member from a previously established black rockfish cDNA database, designating as ‘RfNLRC5’. The complete open reading frame of RfNLRC5 consists of 5808 bp which encodes for a protein of 1936 amino acids with the predicted molecular mass of 213 kDa. Intriguingly, RfNLRC5 harbored only two typical domain signatures of NLR superfamily, namely NACHT domain and LRRs. However, it was phylogenetically closely related to the telostan counterparts. As expected, RfNLRC5 shared significant sequence compatibility with its teleostan counterparts, eminently with that of large yellow croaker. As detected by our qPCR assay, RfNLRC5 was universally distributed in tissues examined, albeit with different levels. Therein, more pronounced expression levels were detected in blood cells and spleen tissues. After treating the naïve fish with immune stimulants; lipopolysaccharides and Polyinosinic:polycytidylic acid (poly I:C), RfNLRC5 mRNA expression in blood cells and spleen tissues was found to modulate significantly with notable inductive responses. Collectively, our results in this study hint a potential role of RfNLRC5 in host innate immune responses against bacterial or viral infections.