Actinomycetes genome engineering approaches

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• 作者：Theresa Siegl (1)
  Andriy Luzhetskyy (2) Andriy.Luzhetskyy@helmholtz-hzi.de
• 关键词：Site ; specific recombinase &#8211 ; Transposon mutagenesis &#8211 ; Reporter genes &#8211 ; Genomic engineering &#8211 ; Actinomycetes
• 刊名：Antonie van Leeuwenhoek
• 出版年：2012
• 出版时间：October 2012
• 年：2012
• 卷：102
• 期：3
• 页码：503-516
• 全文大小：654.5 KB
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• 作者单位：1. Albert-Ludwigs-University of Freiburg, Pharmaceutical Biologie and Biotechnology, Stefan-Meier-st. 19, 79104 Freiburg, Germany2. Helmholtz-Institute for Pharmaceutical Research Saarland, Campus, Building C2 3, 66123 Saarbr眉cken, Germany
• ISSN：1572-9699

文摘

This review provides an overview of new technologies for DNA manipulations in actinomycetes exploiting recombinogenic engineering (Flp-FRT, Cre-loxP, Dre-rox, Tn5, GusA and I-SceI systems). We will describe some new vectors recently developed for engineering of complex phenotypes in actinomycetes. Several site-specific recombinases, transposons, reporter genes and I-SceI endonuclease have been utilized for genome manipulation in actinomycetes. Novel molecular tools will help to overcome many technical difficulties and will encourage new efforts to address the function of actinomycete genes.