The nuclear encoded subunits gamma, delta and epsilon from the shrimp mitochondrial F1-ATP synthase, and their transcriptional response during hypoxia

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• 作者：Oliviert Martinez-Cruz ; Aldo Arvizu-Flores…
• 关键词：Genes ; F1 ATP ; synthase ; Nuclear subunits ; Hypoxia ; Shrimp
• 刊名：Journal of Bioenergetics and Biomembranes
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：47
• 期：3
• 页码：223-234
• 全文大小：3,429 KB
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• 作者单位：Oliviert Martinez-Cruz (1)
  Aldo Arvizu-Flores (1)
  Rogerio R. Sotelo-Mundo (2)
  Adriana Muhlia-Almazan (2)
  
  1. Universidad de Sonora, Rosales y Blvd. Luis Encinas s/n, Hermosillo, Sonora, 83000, Mexico
  2. Centro de Investigacion en Alimentacion y Desarrollo (CIAD), A.C, Carretera a Ejido La Victoria Km 0.6, PO Box. 1735, Hermosillo, Sonora, Mexico
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Bioorganic Chemistry
  Biochemistry
  Animal Anatomy, Morphology and Histology
  Animal Biochemistry
  Organic Chemistry
  
• 出版者：Springer New York
• ISSN：1573-6881

文摘

The mitochondrial F_OF₁ ATP synthase produces ATP in a reaction coupled to an electrochemical proton gradient generated by the electron transfer chain. The enzyme also hydrolyzes ATP according to the energy requirements of the organism. Shrimp need to overcome low oxygen concentrations in water and other energetic stressors, which in turn lead to mitochondrial responses. The aim of this study was to characterize the full-length cDNA sequences of three subunits that form the central stalk of the F₁ catalytic domain of the ATP synthase of the white shrimp Litopenaeus vannamei and their deduced proteins. The effect of hypoxia on shrimp was also evaluated by measuring changes in the mRNA amounts of these subunits. The cDNA sequences of the nucleus-encoded ATPγ, ATPδ and ATPε subunits are 1382, 477 and 277?bp long, respectively. The three deduced amino acid sequences exhibited highly conserved regions when compared to homologous sequences, and specific substitutions found in shrimp subunits are discussed through an homology structural model of F₁ ATP-synthase that included the five deduced proteins, which confirm their functional structures and specific characteristics from the cognate complex of ATP synthases. Genes expression was evaluated during hypoxia-reoxygenation, and resulted in a generalized down-regulation of the F₁ subunits and no coordinated changes were detected among these five subunits. The reduced mRNA levels suggest a mitochondrial response to an oxidative stress event, similar to that observed at ischemia-reperfusion in mammals. This model analysis and responses to hypoxia-reoxygenation may help to better understand additional mitochondrial adaptive mechanisms.