The Gene 4 of Rice Yellow Stunt Rhabdovirus Encodes the Matrix Protein

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• 作者：Zongli Luo ; Xiaoying Chen ; Dongmin Gao and Rongxiang Fang
• 关键词：RYSV ; rhabdovirus ; gene 4 ; nucleotide sequence ; matrix protein
• 刊名：Virus Genes
• 出版年：1998
• 出版时间：1998
• 年：1998
• 卷：16
• 期：3
• 页码：277-280
• 全文大小：79 KB

文摘

The complete nucleotide sequence of the gene 4 of rice yellow stunt rhabdovirus (RYSV) was determined from cDNAs corresponding to the viral genomic RNA. Gene 4 is 913 nucleotides (nt) long, comprising a 17-nt untranslated 5′ region, a 786-nt open reading frame encoding a polypeptide with a molecular mass of 29,125 Da, and a 110-nt untranslated 3′ region. Western blot analysis of the RYSV proteins using the antiserum raised against the protein expressed from the cloned gene in Escherichia coli indicates that gene 4 encodes the M protein of RYSV. Comparisons of the deduced amino acid sequence of the M protein of RYSV with those of other rhabdoviruses revealed no significant homologies. However, it shared a similar basic property and a similar distribution of charges with the other rhabdovirus matrix proteins and showed a relatively closer relationship to the sonchus yellow net virus (SYNV) M1 protein.