Quantitation of biperiden in whole blood by MALDI-QTOF tandem mass spectrometry, and estimation of new metabolites in urine of deceased subjects treated with biperiden antemortem

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• 作者：Kayoko Minakata ; Itaru Yamagishi ; Hideki Nozawa ; Koutaro Hasegawa…
• 关键词：Biperiden ; New metabolites ; MALDI ; QTOF ; tandem mass spectrometry ; Human whole blood and urine
• 刊名：Forensic Toxicology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：35
• 期：1
• 页码：86-93
• 全文大小：
• 刊物主题：Forensic Medicine; Pharmacology/Toxicology; Medicinal Chemistry; Medicine/Public Health, general; Medical Law;
• 出版者：Springer Japan
• ISSN：1860-8973
• 卷排序：35

文摘

In this work, the application of matrix-assisted laser desorption ionization (MALDI)-quadrupole time-of-flight (QTOF)-tandem mass spectrometry (MS/MS) to the quantitation of the anticholinergic/antiparkinson drug biperiden in human whole blood, and the estimation of new phase I metabolites of biperiden in human urine, were performed for the first time. The limit of detection of biperiden in human whole blood was 0.3 ng/mL with a quantitation range of 1–100 ng/mL using 20 μL each of whole blood. The developed method was successfully applied to the determination of biperiden in whole blood obtained from two deceased subjects having a history of biperiden treatment. The concentrations of biperiden in the whole blood samples were 1.7 and 17.5 ng/mL. The MALDI-QTOF-MS/MS method was also used to estimate the metabolites of biperiden in urine obtained from the subjects. Six new metabolites formed by hydroxylation and/or dehydration were detected.