Cellular volume regulation by anoctamin 6: Ca2+, phospholipase A2 and osmosensing

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• 作者：Lalida Sirianant ; Jiraporn Ousingsawat…
• 关键词：TMEM16F ; Anoctamin 6 ; Volume regulation ; Regulatory volume decrease ; RVD ; Volume ; regulated anion channel ; VRAC ; Apoptosis
• 刊名：Pfl¨¹gers Archiv - European Journal of Physiology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：468
• 期：2
• 页码：335-349
• 全文大小：5,350 KB
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• 作者单位：Lalida Sirianant (1)
  Jiraporn Ousingsawat (1)
  Podchanart Wanitchakool (1)
  Rainer Schreiber (1)
  Karl Kunzelmann (1)
  
  1. Institut für Physiologie, Universität Regensburg, Universitätsstraße 31, 93053, Regensburg, Germany
  
• 刊物主题：Human Physiology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1432-2013

文摘

During cell swelling, Cl− channels are activated to lower intracellular Cl− concentrations and to reduce cell volume, a process termed regulatory volume decrease (RVD). We show that anoctamin 6 (ANO6; TMEM16F) produces volume-regulated anion currents and controls cell volume in four unrelated cell types. Volume regulation is compromised in freshly isolated intestinal epithelial cells from Ano6−/− mice and also in lymphocytes from a patient lacking expression of ANO6. Ca2+ influx is activated and thus ANO6 is stimulated during cell swelling by local Ca2+ increase probably in functional nanodomains near the plasma membrane. This leads to stimulation of phospholipase A₂ (PLA₂) and generation of plasma membrane lysophospholipids, which activates ANO6. Direct application of lysophospholipids also activates an anion current that is inhibited by typical ANO6 blocker. An increase in intracellular Ca2+ supports activation of ANO6, but is not required when PLA₂ is fully activated, while re-addition of arachidonic acid completely blocked ANO6. Moreover, ANO6 is activated by low intracellular Cl− concentrations and may therefore operate as a cellular osmosensor. High intracellular Cl− concentration inhibits ANO6 and activation by PLA₂. Taken together, ANO6 supports volume regulation and volume activation of anion currents by action as a Cl− channel or by scrambling membrane phospholipids. Thereby, it may support the function of LRRC8 proteins.