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MiR-21 up-regulation mediates glioblastoma cancer stem cells apoptosis and proliferation by targeting FASLG
- 作者:Chao Shang (1) r> Yan Guo (2) r> Yang Hong (3) r> Yun-hui Liu (3) r> Yi-xue Xue (1) r>r>1. Department of Neurobiology ; China Medical University ; No. 92 Beier Road ; Heping District ; Shenyang ; 110001 ; People鈥檚 Republic of China r> 2. Department of Central Laboratory ; School of Stomatology ; China Medical University ; Shenyang ; 110007 ; People鈥檚 Republic of China r> 3. Department of Neurosurgery ; Shengjing Hospital ; China Medical University ; Shenyang ; 110004 ; People鈥檚 Republic of China r>
- 关键词:Glioblastoma multiforme ; MiR ; 21 ; FASLG ; Cancer stem cells ; U87
- 刊名:Molecular Biology Reports
- 出版年:2015
- 出版时间:March 2015
- 年:2015
- 卷:42
- 期:3
- 页码:721-727
- 全文大小:887 KB
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- 刊物类别:Biomedical and Life Sciences
- 刊物主题:Life Sciencesr>Animal Anatomy, Morphology and Histologyr>Animal Biochemistryr>
- 出版者:Springer Netherlands
- ISSN:1573-4978
文摘
To investigate whether miR-21 can affect the apoptosis and proliferation of glioblastoma cancer stem cells (GSCs) from down-regulating FASLG. The expression of miRNA-21 was detected by quantitative real-time PCR in normal brain tissue and glioblastoma samples, and the changes of miRNA-21 expression between GSCs and non-GSCs were also detected. The apoptosis and proliferation ability of miR-21 in GSCs were analyzed by MTT and flow cytometry assay after anti-miR-21 transfection. For the regulation mechanism analysis of miR-21, TargetScan, PicTar and microRNA were selected to predict some potential target genes of miR-21. The predicted gene was identified to be the direct and specific target gene of miR-21 by luciferase activities assay and western blot. RNA interference technology was used to confirm the apoptosis and proliferation effects of miR-21 were directly induced by FASLG. The expression of miR-21 increased significantly in glioblastoma contrast to normal brain tissue, and miR-21 up-regulated in GSCs remarkably. The proliferation of GSCs cell could be inhibited with high-expression of miR-21 and this effect could be restored by miR-21 knocked down. Mechanism analysis revealed that FASLG was a specific and direct target gene of miR-21. The advanced effects of anti-miR-21 on GSCs apoptosis and proliferation were mediated by expression of silenced FASLG. In summary, aberrantly expressed miR-21 regulates GSCs apoptosis and proliferation partly through directly down-regulating FASLG protein expression in GSCs and this might offer a new potential therapeutic stratagem for glioblastoma.
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