Protection of guinea pigs by vaccination with a recombinant swinepox virus co-expressing HA1 genes of swine H1N1 and H3N2 influenza viruses

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• 作者：Jiarong Xu (1)
  Deji Yang (1)
  Dongyan Huang (1) (2)
  Jiaping Xu (3)
  Shichao Liu (1)
  Huixing Lin (1)
  Haodan Zhu (1)
  Bao Liu (1)
  Chengping Lu (1)
  
• 刊名：Archives of Virology
• 出版年：2013
• 出版时间：March 2013
• 年：2013
• 卷：158
• 期：3
• 页码：629-637
• 全文大小：646KB
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• 作者单位：Jiarong Xu (1)
  Deji Yang (1)
  Dongyan Huang (1) (2)
  Jiaping Xu (3)
  Shichao Liu (1)
  Huixing Lin (1)
  Haodan Zhu (1)
  Bao Liu (1)
  Chengping Lu (1)
  
  1. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China
  2. College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, China
  3. College of Foreign Studies, Nanjing Agricultural University, Nanjing, 210095, China
  
• ISSN：1432-8798

文摘

Swine influenza (SI) is an acute respiratory infectious disease of swine caused by swine influenza virus (SIV). SIV is not only an important respiratory pathogen in pigs but also a potent threat to human health. Here, we report the construction of a recombinant swinepox virus (rSPV/H3-2A-H1) co-expressing hemagglutinin (HA1) of SIV subtypes H1N1 and H3N2. Immune responses and protection efficacy of the rSPV/H3-2A-H1 were evaluated in guinea pigs. Inoculation of rSPV/H3-2A-H1 yielded neutralizing antibodies against SIV H1N1 and H3N2. The IFN-γ and IL-4 concentrations in the supernatant of lymphocytes stimulated with purified SIV HA1 antigen were significantly higher (P?<?0.01) than those of the control groups. Complete protection of guinea pigs against SIV H1N1 or H3N2 challenge was observed. No SIV shedding was detected from guinea pigs vaccinated with rSPV/H3-2A-H1 after challenge. Most importantly, the guinea pigs immunized with rSPV/H3-2A-H1 did not show gross and micrographic lung lesions. However, the control guinea pigs experienced distinct gross and micrographic lung lesions at 7?days post-challenge. Our data suggest that the recombinant swinepox virus encoding HA1 of SIV H1N1 and H3N2 might serve as a promising candidate vaccine for protection against SIV H1N1 and H3N2 infections.