Plant regeneration through direct somatic embryogenesis from immature zygotic embryos of the medicinal plant, Paris polyphylla Sm.

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• 作者：Shiveirou Raomai (1)
  Suman Kumaria (1)
  Pramod Tandon (1)
  
• 关键词：Conservation ; Direct somatic embryogenesis ; Endangered ; Immature zygotic embryos ; Medicinal plant ; Paris polyphylla
• 刊名：Plant Cell, Tissue and Organ Culture
• 出版年：2014
• 出版时间：September 2014
• 年：2014
• 卷：118
• 期：3
• 页码：445-455
• 全文大小：1,749 KB
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• 作者单位：Shiveirou Raomai (1)
  Suman Kumaria (1)
  Pramod Tandon (1)
  
  1. Plant Biotechnology Laboratory, Department of Botany, Centre for Advanced Studies, North-Eastern Hill University, Shillong, 793022, India
  
• ISSN：1573-5044

文摘

A protocol for induction of direct somatic embryogenesis and subsequent plant regeneration for the medicinally important and endangered plant Paris polyphylla Sm. has been developed for the first time. Immature zygotic embryos (IZEs) were cultured on different media namely Gamborg (B5), ? B5, Murashige and Skoog (MS), ? MS, Chu et al. (N6), ? N6, Schenk and Hildebrandt (SH) and ? SH. Highest frequency of somatic embryogenesis (32.6?%) and mean number of somatic embryos (SEs) per explant (28.7?±?1.7) were obtained on ? MS medium directly without an intermediate callus phase. The frequency of SE induction was significantly increased to 40.7?% when ? MS medium was solidified with gelrite compared to agar (32.6?%). Secondary somatic embryos (SSEs) appeared on the primary SEs in a repetitive way on plant growth regulator-free ? MS medium but with a gradual decrease in embryogenic potential during subsequent subcultures. Plasmolyzing pre-treatment of SSEs with 1.0?M mannitol for 12?h effectively maintains its embryogenic capacity. Primary embryos at the elongated dimpled and early cotyledonary stage displayed the highest embryo forming capacity of 26.94 and 27.87, respectively. High frequency of SE germination (94.0?%) occurred on ? MS medium with 0.5?mg/l gibberellic acid. Highest percentage of seedling to plantlet conversion was observed in the medium supplemented with 0.05?mg/l 6-benzylaminopurine and 0.1?mg/l α-naphthalene acetic acid. Regenerated plants displayed morphological characteristics similar to that of the wild plants. Flow cytometry analysis showed ploidy stability of the regenerated plants.