Germplasm resources research of Toona sinensis with RAPD and isoenzyme analysis

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• 作者：Changlu Wang (1)
  Junwu Cao (2)
  Shaoran Tian (2)
  Yurong Wang (1)
  Zhiqiang Chen (1)
  Mianhua Chen (1)
  Guoli Gong (1)
  
• 关键词：Toona sinensis ; RAPD ; germplasm resources ; isoenzyme analysis
• 刊名：Biologia
• 出版年：2008
• 出版时间：June 2008
• 年：2008
• 卷：63
• 期：3
• 页码：320-326
• 全文大小：307KB
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• 作者单位：Changlu Wang (1)
  Junwu Cao (2)
  Shaoran Tian (2)
  Yurong Wang (1)
  Zhiqiang Chen (1)
  Mianhua Chen (1)
  Guoli Gong (1)
  
  1. College of Food Engineering & Biotechnology, Tianjin University of Science & Technology, Tianjin, 300457, People鈥檚 Republic of China
  2. College of Bioengineering, Tianjin University of Science & Technology, Tianjin, 300457, People鈥檚 Republic of China
  

文摘

Collected from Chinese mainland, 25 samples of Toona sinensis seed were planted in a standard garden. Three years later, samples were analyzed with random amplified polymorphic DNA (RAPD) and isoenzymes variants of cytochrome oxidase and peroxidases. Results of RAPD and isoenzymes variants were analyzed with software POPGENE32 and SPSS10.0 respectively. Phylogenetic trees were calculated from the clustered result of RAPD and isoenzymes variants. Such additional factors as leaves color, latitude and altitude of original seeds were analyzed at the same time. Our result suggested that a general similarity is concurred with the three analyses as RAPD, isoenzymes variants analysis of peroxidases and cytochrome oxidase. Two clusters can be separated from the sample collected as southern and northern ones. In the evolutionary history, a migrating route in the direction from West-South to East-North and with sequential differentiation in the direction South-North can be decided if only it is true with the argument that Toona sinensis came from tropic Asia, the border of east-north India and Burma.