A metallo-keratinase from a newly isolated Acinetobacter sp. R-1 with low collagenase activity and its biotechnological application potential in leather industry

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• 作者：Rong-Xian Zhang ; Jin-Song Gong ; Dan-Dan Zhang…
• 关键词：Acinetobacter sp. R ; 1 ; Keratinase ; Purification ; Dehairing ; Surface modification
• 刊名：Bioprocess and Biosystems Engineering
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：39
• 期：1
• 页码：193-204
• 全文大小：1,123 KB
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• 作者单位：Rong-Xian Zhang (1) (2)
  Jin-Song Gong (1)
  Dan-Dan Zhang (1)
  Chang Su (1)
  Ying-Shuo Hou (1)
  Heng Li (1)
  Jin-Song Shi (1)
  Zheng-Hong Xu (1) (2)
  
  1. School of Pharmaceutical Science, Jiangnan University, Wuxi, 214122, People’s Republic of China
  2. The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, People’s Republic of China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Industrial Chemistry and Chemical Engineering
  Industrial and Production Engineering
  Waste Management and Waste Technology
  Waste Water Technology, Water Pollution Control, Water Management and Aquatic Pollution
  Food Science
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1615-7605

文摘

Microbial keratinase is a well-recognized enzyme that can specifically degrade insoluble keratins. A keratinase-producing bacterium was isolated from a duck ranch soil and identified as Acinetobacter sp. R-1 based on the biochemical characteristics and 16S rDNA gene sequencing. It showed high keratinase activity and low collagenase activity. The keratinase was purified to electrophoretic homogeneity with 6.69 % recovery, 2.68-fold purification and an estimated molecular weight of 25 kDa. Additionally, the keratinase showed optimal activity at 50 °C and pH11. Keratinase activity of Acinetobacter sp. significantly increased in the presence of Li+, Na+, and Ca2+, while it was completely inhibited by EDTA, indicating it was a metallo-keratinase. Moreover, the crude keratinase from Acinetobacter sp. R-1 could thoroughly depilate goat skin and simultaneously modify the wool surface, which indicated its applicable potential in leather and textile industries.