Differential response related to genotoxicity in multiple organs of cirrhotic rats

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• 作者：Marcia R. Nagaoka (1)
  Luciana Le Sueur-Maluf (1)
  Odair Aguiar Jr (1)
  Glaucia M. Castro (1)
  Regina C. Spadari-Bratfisch (1)
  Daniel A. Ribeiro (1)
  
• 关键词：Cirrhosis ; Rats ; DNA damage ; Single cell gel (comet) assay
• 刊名：Hepatology International
• 出版年：2011
• 出版时间：June 2011
• 年：2011
• 卷：5
• 期：2
• 页码：740-746
• 全文大小：508KB
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• 作者单位：Marcia R. Nagaoka (1)
  Luciana Le Sueur-Maluf (1)
  Odair Aguiar Jr (1)
  Glaucia M. Castro (1)
  Regina C. Spadari-Bratfisch (1)
  Daniel A. Ribeiro (1)
  
  1. Departamento de Biociências, Universidade Federal de Sao Paulo, UNIFESP, Santos, SP, Brazil
  

文摘

Purpose The aim of this study was to use the single cell gel (comet) assay to investigate whether blood, liver, heart, kidney, and brain are particularly sensitive organs for DNA damage in cirrhotic rats to predict genetic instability induced by cirrhosis. Methods A total of 16 male Wistar rats (negative control, n?=?8; experimental, n?=?8) were submitted to bile duct ligation during 28?days. Results Cirrhosis was able to induce genetic damage in liver and brain cells, as depicted by the mean tail moment. No genetic damage was induced in blood, heart, or kidney cells (i.e., no significant statistically differences were noticed when compared with negative control). Conclusions In conclusion, our results suggest that cirrhosis could contribute to DNA damage in liver and brain cells.