Confocal stereology: an efficient tool for measurement of microscopic structures

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• 作者：Lucie Kubínová ; Ji?í Janá?ek
• 关键词：3 ; D images ; Confocal microscopy ; Geometrical characteristics ; Spatial probes ; Stereology
• 刊名：Cell and Tissue Research
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：360
• 期：1
• 页码：13-28
• 全文大小：2,018 KB
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• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Human Genetics
  Proteomics
  Molecular Medicine
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0878

文摘

Quantitative measurements of geometric forms or counting of objects in microscopic specimens is an essential tool in studies of microstructure. Confocal stereology represents a contemporary approach to the evaluation of microscopic structures by using a combination of stereological methods and confocal microscopy. 3-D images acquired by confocal microscopy can be used for the estimation of geometrical characteristics of microscopic structures by stereological methods, based on the evaluation of optical sections within a thick slice and using computer-generated virtual test probes. Such methods can be used for estimating volume, number, surface area and length using relevant spatial probes, which are generated by specific software. The interactions of the probes with the structure under study are interactively evaluated. An overview of the methods of confocal stereology developed during the past 30?years is presented. Their advantages and pitfalls in comparison with other methods for measurement of geometrical characteristics of microscopic structures are discussed.