Stool Methylated DNA Markers Decrease Following Colorectal Cancer Resection—Implications for Surveillance

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• 作者：John B. Kisiel (1)
  Tracy C. Yab (1)
  William R. Taylor (1)
  Douglas W. Mahoney (2)
  David A. Ahlquist (1)
  
• 关键词：Colorectal neoplasms ; Feces analysis ; Secondary prevention ; DNA methylation
• 刊名：Digestive Diseases and Sciences
• 出版年：2014
• 出版时间：August 2014
• 年：2014
• 卷：59
• 期：8
• 页码：1764-1767
• 全文大小：187 KB
• 参考文献：1. Levin B, Lieberman DA, McFarland B, et al. Screening and surveillance for the early detection of colorectal cancer and adenomatous polyps, 2008: a joint guideline from the American cancer society, the US multi-society task force on colorectal cancer, and the American college of radiology. / Gastroenterology. 2008;134:1570-595. CrossRef
  2. Rex DK, Johnson DA, Anderson JC, Schoenfeld PS, Burke CA, Inadomi JM. American college of gastroenterology guidelines for colorectal cancer screening 2009 [corrected]. / Am J Gastroenterol. 2009;104:739-50. CrossRef
  3. Shen L, Kondo Y, Rosner GL, et al. Mgmt promoter methylation and field defect in sporadic colorectal cancer. / J Natl Cancer Inst. 2005;97:1330-338. CrossRef
  4. Edge SBB, Compton CC, Fritz AG, Greene FL, Trotti A, eds. / AJCC cancer staging manual. 7th ed. New York: Springer; 2010:646.
  5. Rex DK, Kahi CJ, Levin B, et al. Guidelines for colonoscopy surveillance after cancer resection: a consensus update by the American cancer society and the US multi-society task force on colorectal cancer. / Gastroenterology. 2006;130:1865-871. CrossRef
  6. Lidgard GP, Domanico M, Bruinsma JJ, et al. An optimized multi-marker stool test for colorectal cancer screening: Initial clinical appraisal. / Gastroenterology. 2012;142:S-770.
  7. Lidgard GP, Domanico MJ, Bruinsma JJ, et al. Clinical performance of an automated stool DNA assay for detection of colorectal neoplasia. / Clin Gastroenterol Hepatol. 2013;11:1313-318. CrossRef
  8. Imperiale TF, Ransohoff DF, Itzkowitz SH, et al. Multitarget stool DNA testing for colorectal-cancer screening. / New Engl J Med. 2014;370:1287-297. CrossRef
  9. Syngal S, Stoffel E, Chung D, et al. Detection of stool DNA mutations before and after treatment of colorectal neoplasia. / Cancer. 2006;106:277-83. CrossRef
  10. Zou H, Allawi H, Cao X, et al. Quantification of methylated markers with a multiplex methylation-specific technology. / Clin Chem. 2012;58:375-83. CrossRef
  11. Ahlquist DA, Zou H, Domanico M, et al. Next-generation stool DNA test accurately detects colorectal cancer and large adenomas. / Gastroenterology. 2012;142:248-56. CrossRef
  12. Ahlquist DA, Taylor WR, Yab TC, et al. Aberrantly methylated gene marker levels in stool: effect of demographic exposure, body mass, and other patient characteristics. / J Mol Biomark Diagn. 2012;3:133.
  
• 作者单位：John B. Kisiel (1)
  Tracy C. Yab (1)
  William R. Taylor (1)
  Douglas W. Mahoney (2)
  David A. Ahlquist (1)
  
  1. Division of Gastroenterology and Hepatology, Mayo Clinic, 200 First Street Southwest, Rochester, MN, 55905, USA
  2. Biomedical Statistics and Informatics, Mayo Clinic, Rochester, MN, USA
  
• ISSN：1573-2568

文摘

Background Molecular changes associated with colorectal cancer (CRC) are detected by stool deoxyribonucleic acid testing but could persist following tumor resection. Aims We sought to determine whether methylated gene markers in stool normalize after CRC resection. Methods We studied stools from 22 CRC cases before and after subtotal resection and from 80 colonoscopy-normal controls. In blinded fashion, target genes (methylated NDRG4 and BMP3) were captured from stool supernatant, bisulfite-treated, and assayed by quantitative allele-specific real-time target and signal amplification. Results were dichotomized at 95?% specificity cutoffs. Results Among CRC cases, median methylated NDRG4 and BMP3 levels decreased dramatically (4- to 15-fold) following resection, p?=?0.003 and p?p?=?0.0002. A case whose stool methylated NDRG4 level increased sharply after surgery was found to have recurrent CRC. Conclusions Methylated gene marker levels clear from stool following CRC resection unless disease is present. Postoperative stool marker levels are informative and may be of value in surveillance.