Electrochemical aptasensor for tetracycline using a screen-printed carbon electrode modified with an alginate film containing reduced graphene oxide and magnetite (Fe3O4) nanoparticles

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• 作者：Xuejia Zhan ; Guangzhi Hu ; Thomas Wagberg ; Shenshan Zhan ; Hanchu Xu…
• 关键词：Cyclic voltammetry ; Differential pulse voltammetry ; Scanning electron microscopy ; Transmission electron microscopy ; Sodium alginate ; Thionine ; Redox probe
• 刊名：Microchimica Acta
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：183
• 期：2
• 页码：723-729
• 全文大小：894 KB
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• 作者单位：Xuejia Zhan (1)
  Guangzhi Hu (2) (3)
  Thomas Wagberg (3)
  Shenshan Zhan (1)
  Hanchu Xu (1)
  Pei Zhou (1)
  
  1. School of Agriculture and Biology & Bor S. Luh Food Safety Research Center; Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai Jiaotong University, Shanghai, 200240, People’s Republic of China
  2. Laboratory of Environmental Science and Technology, The Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, 830011, People’s Republic of China
  3. Department of Physics, Umea University, 901 87, Umea, Sweden
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Inorganic Chemistry
  Physical Chemistry
  Characterization and Evaluation Materials
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Wien
• ISSN：1436-5073

文摘

The authors describe a label-free electrochemical aptasensor for tetracycline (TET). The TET-binding aptamer was immobilized on a composite consisting of reduced graphene oxide, magnetite (Fe₃O₄) and sodium alginate, and this material was used to modify the surface of a screen-printed carbon electrode (SPCE). Cyclic voltammetry was carried out to characterize the single steps in the preparation of the modified electrode and to optimize the conditions for detection. Differential pulse voltammetry (DPV) was then used to monitor the interaction between aptamer and TET by applying the electrochemical probe thionine. Under optimal conditions, TET can be quantified by DPV in the 1 nM to 5 μM concentration range, with a detection limit as low as 0.6 nM (at an S/N ratio of 3). The method is rapid, cost-efficient, highly sensitive and specific, and therefore is considered to be a viable platform for TET analysis in food, environmental, and clinical samples.