Thionin-like peptide from Capsicum annuum fruits: mechanism of action and synergism with fluconazole against Candida species

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• 作者：Gabriel B. Taveira ; André O. Carvalho ; Rosana Rodrigues…
• 关键词：Antimicrobial peptides ; Thionin ; Synergistic activity ; Fluconazole ; Candida
• 刊名：BMC Microbiology
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：16
• 期：1
• 全文大小：890 KB
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• 作者单位：Gabriel B. Taveira (1)
  André O. Carvalho (1)
  Rosana Rodrigues (2)
  Fernanda G. Trindade (3)
  Maura Da Cunha (3)
  Valdirene M. Gomes (1)
  
  1. Laboratório de Fisiologia e Bioquímica de Microrganismos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, 28013-602,, RJ, Brazil
  2. Laboratório de Melhoramento Genético Vegetal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, 28013-602,, RJ, Brazil
  3. Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, 28013-602,, RJ, Brazil
  
• 刊物主题：Microbiology; Biological Microscopy; Fungus Genetics; Parasitology; Virology; Life Sciences, general;
• 出版者：BioMed Central
• ISSN：1471-2180

文摘

Background Thionins are a family of plant antimicrobial peptides (AMPs), which participate in plant defense system against pathogens. Here we describe some aspects of the CaThi thionin-like action mechanism, previously isolated from Capsicum annuum fruits. Thionin-like peptide was submitted to antimicrobial activity assays against Candida species for IC₅₀ determination and synergism with fluconazole evaluation. Viability and plasma membrane permeabilization assays, induction of intracellular ROS production analysis and CaThi localization in yeast cells were also investigated.