Red Sea Atlantis II brine pool nitrilase with unique thermostability profile and heavy metal tolerance

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• 作者：Sarah A. Sonbol ; Ari J. S. Ferreira ; Rania Siam
• 关键词：Nitrilase ; Atlantis II Deep brine pool ; Red Sea ; Metagenomics ; Heavy metals tolerance ; Thermostability
• 刊名：BMC Biotechnology
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：16
• 期：1
• 全文大小：2,203 KB
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• 作者单位：Sarah A. Sonbol (1)
  Ari J. S. Ferreira (1)
  Rania Siam (1)
  
  1. Biology Department and YJ-Science and Technology Research Center, American University in Cairo, New Cairo, 11835, Egypt
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Biotechnology
  Life Sciences
  Plant Breeding/Biotechnology
  Stem Cells
  Transgenics
  
• 出版者：BioMed Central
• ISSN：1472-6750

文摘

Background Nitrilases, which hydrolyze nitriles in a one-step reaction into carboxylic acids and ammonia, gained increasing attention because of the abundance of nitrile compounds in nature and their use in fine chemicals and pharmaceutics. Extreme environments are potential habitats for the isolation and characterization of extremozymes including nitrilases with unique resistant properties. The Red Sea brine pools are characterized by multitude of extreme conditions. The Lower Convective Layer (LCL) of the Atlantis II Deep Brine Pool in the Red Sea is characterized by elevated temperature (68 °C), high salt concentrations (250 ‰), anoxic conditions and high heavy metal concentrations.