Genome sequencing reveals mechanisms for heavy metal resistance and polycyclic aromatic hydrocarbon degradation in Delftia lacustris strain LZ-C

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• 作者：Wenyang Wu ; Haiying Huang ; Zhenmin Ling ; Zhengsheng Yu ; Yiming Jiang…
• 关键词：Delftia lacustris ; Genome sequencing ; Heavy metal resistance ; Polycyclic aromatic hydrocarbons
• 刊名：Ecotoxicology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：25
• 期：1
• 页码：234-247
• 全文大小：1,432 KB
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• 作者单位：Wenyang Wu (1)
  Haiying Huang (1)
  Zhenmin Ling (1)
  Zhengsheng Yu (1)
  Yiming Jiang (1)
  Pu Liu (1)
  Xiangkai Li (1)
  
  1. MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, Gansu, People’s Republic of China
  
• 刊物类别：Earth and Environmental Science
• 刊物主题：Environment
  Environment
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  Ecology
  Environmental Management
  
• 出版者：Springer Netherlands
• ISSN：1573-3017

文摘

Strain LZ-C, isolated from a petrochemical wastewater discharge site, was found to be resistant to heavy metals and to degrade various aromatic compounds, including naphenol, naphthalene, 2-methylnaphthalene and toluene. Data obtained from 16S rRNA gene sequencing showed that this strain was closely related to Delftia lacustris. The 5,889,360 bp genome of strain LZ-C was assembled into 239 contigs and 197 scaffolds containing 5855 predicted open reading frames (ORFs). Among these predicted ORFs, 464 were different from the type strain of Delftia. The minimal inhibitory concentrations were 4 mM, 30 µM, 2 mM and 1 mM for Cr(VI), Hg(II), Cd(II) and Pb(II), respectively. Both genome sequencing and quantitative real-time PCR data revealed that genes related to Chr, Czc and Mer family genes play important roles in heavy metal resistance in strain LZ-C. In addition, the Na+/H+ antiporter NhaA is important for adaptation to high salinity resistance (2.5 M NaCl). The complete pathways of benzene and benzoate degradation were identified through KEGG analysis. Interestingly, strain LZ-C also degrades naphthalene but lacks the key naphthalene degradation gene NahA. Thus, we propose that strain LZ-C exhibits a novel protein with a function similar to NahA. This study is the first to reveal the mechanisms of heavy metal resistance and salinity tolerance in D. lacustris and to identify a potential 2-methylnaphthalene degradation protein in this strain. Through whole-genome sequencing analysis, strain LZ-C might be a good candidate for the bioremediation of heavy metals and polycyclic aromatic hydrocarbons.