Are Rod Outer Segment ATP-ase and ATP-Synthase Activity Expression of the Same Protein?

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• 作者：Daniela Calzia (1)
  Simona Candiani (2)
  Greta Garbarino (2)
  Federico Caicci (3)
  Silvia Ravera (1)
  Maurizio Bruschi (4)
  Lucia Manni (3)
  Alessandro Morelli (1)
  Carlo Enrico Traverso (5)
  Giovanni Candiano (4)
  Carlo Tacchetti (6)
  Isabella Panfoli (1)
  
• 关键词：ATPase ; F1Fo ; ATP synthase ; IF1 ; Rod outer segments
• 刊名：Cellular and Molecular Neurobiology
• 出版年：2013
• 出版时间：July 2013
• 年：2013
• 卷：33
• 期：5
• 页码：637-649
• 全文大小：775KB
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• 作者单位：Daniela Calzia (1)
  Simona Candiani (2)
  Greta Garbarino (2)
  Federico Caicci (3)
  Silvia Ravera (1)
  Maurizio Bruschi (4)
  Lucia Manni (3)
  Alessandro Morelli (1)
  Carlo Enrico Traverso (5)
  Giovanni Candiano (4)
  Carlo Tacchetti (6)
  Isabella Panfoli (1)
  
  1. DIFAR, Universit脿 di Genova, Genoa, Italy
  2. DISTAV, Universit脿 di Genova, Genoa, Italy
  3. Dipartimento di Biologia, Universit脿 di Padova, Padua, Italy
  4. Laboratorio di Fisiopatologia dell鈥橴remia, Istituto G. Gaslini, Genoa, Italy
  5. Clinica Oculistica-DINOGMI, Universit脿 di Genova, Genoa, Italy
  6. IFOM Centro di Oncologia cellulare e Ultrastruttura, Dipartimento di Medicina Sperimentale, Universit脿 degli Studi di Genova, Genoa, Italy
  
• ISSN：1573-6830

文摘

Vertebrate retinal rod outer segments (OS) consist of a stack of disks surrounded by the plasma membrane, where phototransduction takes place. Energetic metabolism in rod OS remains obscure. Literature described a so-called Mg2+-dependent ATPase activity, while our previous results demonstrated the presence of oxidative phosphorylation (OXPHOS) in OS, sustained by an ATP synthetic activity. Here we propose that the OS ATPase and ATP synthase are the expression of the same protein, i.e., of F1Fo-ATP synthase. Imaging on bovine retinal sections showed that some OXPHOS proteins are expressed in the OS. Biochemical data on bovine purified rod OS, characterized for purity, show an ATP synthase activity, inhibited by classical F1Fo-ATP synthase inhibitors. Moreover, OS possess a pH-dependent ATP hydrolysis, inhibited by pH values below 7, suggestive of the functioning of the inhibitor of F1 (IF1) protein. WB confirmed the presence of IF1 in OS, substantiating the expression of F1Fo ATP synthase in OS. Data suggest that the OS F1Fo ATP synthase is able to hydrolyze or synthesize ATP, depending on in vitro or in vivo conditions and that the role of IF1 would be pivotal in the prevention of the reversal of ATP synthase in OS, for example during hypoxia, granting photoreceptor survival.