Ochrobactrum sp. Pv2Z2 exhibits multiple traits of plant growth promotion, biodegradation and N-acyl-homoserine-lactone quorum sensing

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• 作者：Asma Imran (1)
  Marryam Jumma Abdulla Saadalla (1) (2)
  Sami-Ullah Khan (1)
  Muhammad Sajjad Mirza (1)
  Kauser Abdulla Malik (1) (3)
  Fauzia Yusuf Hafeez (1) (4)
  
• 关键词：Legumes ; Ochrobactrum ; Biodegradation ; Phosphate solubilization ; AHLs
• 刊名：Annals of Microbiology
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：64
• 期：4
• 页码：1797-1806
• 全文大小：896 KB
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  13. Chakraborty U, Chakraborty BN, Basnet M, Chakraborty AP (2009) Evaluation of / Ochrobactrum anthropi TRS-2 and its talc based formulation for enhancement of growth of tea plants and management of brown root rot disease. J Appl Microbiol 107:625-34 CrossRef
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• 作者单位：Asma Imran (1)
  Marryam Jumma Abdulla Saadalla (1) (2)
  Sami-Ullah Khan (1)
  Muhammad Sajjad Mirza (1)
  Kauser Abdulla Malik (1) (3)
  Fauzia Yusuf Hafeez (1) (4)
  
  1. National Institute for Biotechnology and Genetic Engineering, P.O. Box 577, Jhang Road, Faisalabad, Pakistan
  2. Ministry of Agriculture, Natural Resources, Environment and Co-operatives, Zanzibar, Tanzania
  3. FC College University, Lahore, Pakistan
  4. COMSTAS Institute of Information Technology, Islamabad, Pakistan
  
• ISSN：1869-2044

文摘

Rhizosphere bacteria play a vital role in plant growth, pathogen control, biodegradation and rhizosphere signaling. A motile, rod-shaped bacterium, Pv2Z2, isolated from the nodules of the common bean grown in Tanzanian soil was characterized using a polyphasic approach. The traits assessed included the production of indole-3-acetic acid and N-acyl homoserine lactone (AHL) molecules, solubilization of insoluble phosphate and zinc compounds and biodegradation of a number of toxic compounds. The 16S rRNA sequence of Pv2Z2 (EU399793) showed 99?% homology to Ochrobactrum anthropi isolates (Accession no. AJ867292, AJ867291, AJ867290) from soil samples of wheat root. Phylogenetic analysis showed relatedness to nodulating strain Ochrobactrum cytisi rather than to the clinical/pathogenic type strain of O. anthropi. Moreover, it showed unique fingerprints in the randomly amplified polymorphic DNA (RAPD) and two primers-RAPD assays which were different from those of the pathogenic type strain of O. anthropi. The bacterium produced 6.68?μg/mL-1 indoleacetic acid in the presence of tryptophan, released 25.7?μg/mL-1 phosphorus from inorganic tri-calcium phosphate in the Pikoviskaya’s medium and solubilized zinc sulphate and zinc oxide in the LG1 medium. The production of AHLs (e.g. 3O-C7-HSL, 3OH-C7-HSL) was detected with biosensor strains CV026 using reverse phase thin layer chromatography. The bacterium was able to grow in minimal salt medium supplemented with 100?mg/L each of phenol, 2-bromophenol, 2,4-diamino phenol hydrochloride, 3,4-dimethoxy benzyl alcohol and 4-methoxy benzyl alcohol. Phenol degradation was recorded up to a level of 94?% within 12?days. Inoculation of common bean plants resulted in a significant increase in plant height, fresh/dry weight and nitrogen uptake as compared to non-inoculated plants. The data suggest that the plant growth-promoting and biodegradation potential of this bacterium may be exploited on a large scale. The capacity to produce AHL molecules by members of the Ochrobactrum genus has not been previously reported and needs to be explored in detail.