Human hair keratin-based biofilm for potent application to periodontal tissue regeneration

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• 作者：Hanna Lee (1)
  Yu-Shik Hwang (2)
  Hyo-Seol Lee (3)
  Suji Choi (2)
  So Yeon Kim (2)
  Ji-Hoi Moon (2)
  Jae Hyung Kim (3)
  Kwang Chul Kim (2)
  Dong-Wook Han (4)
  Hyun-Jin Park (1)
  Hojae Bae (5)
  
  1. Department of Food Biochemistry and Engineering ; Korea University ; Seoul ; 136-713 ; Korea
  2. Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology ; School of Dentistry ; Kyung Hee University ; Seoul ; 130-701 ; Korea
  3. Department of Pediatric Dentistry ; School of Dentistry ; Kyung Hee University ; Seoul ; 130-701 ; Korea
  4. Department of Cogno-Mechatronics Engineering ; Pusan National University ; Busan ; 609-735 ; Korea
  5. Department of Bioindustrial Technologies ; College of Animal Bioscience and Technology ; Konkuk University ; Hwayang-dong ; Gwangjin-gu ; Seoul ; 143-701 ; Korea
  
• 关键词：human hair keratin ; film ; drug ; eluting ; periodontal tissue
• 刊名：Macromolecular Research
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：23
• 期：3
• 页码：300-308
• 全文大小：786 KB
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Physical Chemistry
  Polymer Sciences
  Characterization and Evaluation of Materials
  Soft and Granular Matter, Complex Fluids and Microfluidics
  Nanochemistry
  Nanotec
  
• 出版者：The Polymer Society of Korea, co-published with Springer
• ISSN：2092-7673

文摘

For the treatment of periodontitis, local delivery of antibiotics and their sustained release are preferable to enhance microbial susceptibility and to reduce possible side effects occurrence in systemic drug delivery. To address these issues, antibiotic loaded polymeric matrices implantable into periodontal pocket have been commercially used in dentistry and oral medicine. Recently, keratin has been drawing attention as a natural polymer for its ability to mediate cell behavior with minor or no immunogenicity enabling further process towards autologous implantation. In this study, human hair keratin was extracted with a cocktail of reducing agents, and antibiotic eluting keratin-based biofilms were fabricated. Physicochemical analysis and release test showed proper physical stability and sustained release of the loaded antibiotics. In addition, the released antibiotic suppressed the growth of various types of oral bacteria including porphyromonas gingivalis. Cellular interaction studies showed that human oral epithelial cell, human gingival fibroblast and periodontal ligament cells proliferated and guided well on biofilms. This study propose that antibiotic eluting keratin-based biofilms are provisional device for the treatment of chronic periodontitis offering advantages such as local controlled drug delivery and biocompatibility, and human hair keratin is able to be a good biomaterial for the potent applications to tissue engineering and regenerative medicine.