Neural Differentiation of Mesenchymal Stem Cells Influences Their Chemotactic Responses to Stromal Cell-Derived Factor-1α

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• 作者：Xiaojing Xu ; Guiqin Xie ; Ya’nan Hu ; Xianyang Li…
• 关键词：Mesenchymal stem cells (MSCs) ; Stromal cell ; derived factor ; 1α (SDF ; 1α) ; Chemotactic response ; Migration ; Neural differentiation
• 刊名：Cellular and Molecular Neurobiology
• 出版年：2014
• 出版时间：October 2014
• 年：2014
• 卷：34
• 期：7
• 页码：1047-1058
• 全文大小：3,231 KB
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• 作者单位：Xiaojing Xu (1)
  Guiqin Xie (1)
  Ya’nan Hu (1)
  Xianyang Li (1)
  Ping Huang (1)
  Huanxiang Zhang (1)
  
  1. Jiangsu Key Laboratory of Stem Cell Research, Department of Cell Biology, Medical College of Soochow University, Ren Ai Road 199, Suzhou Industrial Park, Suzhou, 215123, China
  
• ISSN：1573-6830

文摘

Mesenchymal stem cells (MSCs) are proposed as a promising source for cell-based therapies in neural disease. Although increasing numbers of studies have been devoted to the delineation of factors involved in the migration of MSCs, the relationship between the chemotactic response and the differentiation status of these cells is still unclear. In the present study, we demonstrated that MSCs in varying neural differentiation states display various chemotactic responses to stromal cell-derived factor-1α (SDF-1α). The chemotactic responses of MSCs under different differentiation stages in response to SDF-1α were analyzed by Boyden chamber, and the results showed that cells of undifferentiation, 24-h preinduction, 5-h induction, and 18-h maintenance states displayed a stronger chemotactic response to SDF-1α, while 48-h maintenance did not. Further, we found that the phosphorylation levels of PI3K/Akt, ERK1/2, SAPK/JNK, and p38MAPK are closely related to the differentiation states of MSCs subjected to SDF-1α, and finally, inhibition of SAPK/JNK signaling significantly attenuates SDF-1α-stimulated transfilter migration of MSCs of undifferentiation, 24-h preinduction, 18-h maintenance, and 48-h maintenance, but not MSCs of 5-h induction. Meanwhile, interference with PI3K/Akt, p38MAPK, or ERK1/2 signaling prevents only cells at certain differentiation state from migrating in response to SDF-1α. Collectively, these results demonstrate that MSCs in varying neural differentiation states have different migratory capacities, thereby illuminating optimization of the therapeutic potential of MSCs to be used for neural regeneration after injury.