Development of microsatellite markers for genetic analysis in the large scale loach Paramisgurnus dabryanus

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• 作者：Zhiguo Dong (1)
  Xiaoying Li (1)
  Xiaojun Zhang (1)
  Qufei Ling (2)
  Qiangan Ni (3)
  Yong Li (3)
  
• 关键词：Paramisgurnus dabryanus ; Large scale loach ; Microsatellites ; Genetic characterization ; Conservation biology
• 刊名：Conservation Genetics Resources
• 出版年：2014
• 出版时间：March 2014
• 年：2014
• 卷：6
• 期：1
• 页码：151-153
• 全文大小：148 KB
• 作者单位：Zhiguo Dong (1)
  Xiaoying Li (1)
  Xiaojun Zhang (1)
  Qufei Ling (2)
  Qiangan Ni (3)
  Yong Li (3)
  
  1. Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang, 222005, Jiangsu Province, China
  2. Fisheries Research Institute of Soochow University, Suzhou, 215123, Jiangsu Province, China
  3. Siyang Fishery Technical Guidance Station, Suzhou, 222100, Jiangsu, China
  
• ISSN：1877-7260

文摘

The large scale loach (Paramisgurnus dabryanus) is endemic to China and is one of the most abundant edible fish in East Asia. The species is invasive in Japan, competing and hybridizing with the native Misgurnus anguillicaudatus. In this study, microsatellites were developed for the first time in this species using biotinylated probes for hybridization and library screening. Twelve polymorphic microsatellite loci were isolated and characterized in farmed fish. The number of alleles varied between two and five, and the observed and expected heterozygosity ranged from 0.32 to 1.00 and 0.51 to 0.75, respectively. Three loci (Pda14-102, Pda15-139, Pda18-104) deviated significantly from Hardy–Weinberg equilibrium values (P?<?0.01). These microsatellite markers could prove useful for genetic identification and population management of P. dabryanus in the future.