Bm59 is an early gene, but is unessential for the propagation and assembly of Bombyx mori nucleopolyhedrovirus

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• 作者：Xiaolong Hu ; Yunwang Shen ; Qin Zheng ; Guobao Wang…
• 关键词：Bombyx mori nucleopolyhedrovirus (BmNPV) ; Bm59 gene ; Early gene
• 刊名：Molecular Genetics and Genomics
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：291
• 期：1
• 页码：145-154
• 全文大小：1,406 KB
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• 作者单位：Xiaolong Hu (1) (2)
  Yunwang Shen (1)
  Qin Zheng (1)
  Guobao Wang (1)
  Xiaofeng Wu (1)
  Chengliang Gong (2)
  
  1. College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China
  2. School of Biology and Basic Medical Science, Soochow University, Suzhou, 215123, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Biochemistry
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1617-4623

文摘

Bombyx mori nucleopolyhedrovirus (BmNPV) is a major pathogen that specifically infects the domestic silkworm and causes serious economic loss to sericulture around the world. The function of BmNPV Bm59 gene in the viral life cycle is inconclusive. To investigate the role of Bm59 during viral infection, the transcription initiation site and temporal expression of Bm59 were analyzed, and Bm59-knockout virus was generated through homologous recombination in Escherichia coli. The results showed that Bm59 is an early transcription gene with an atypia early transcriptional start motif. Budded virion (BV) production and DNA replication in the BmN cells transfected with the Bm59-knockout virus bacmid were similar to those in the cells transfected with the wild-type virus. Electron microscopy revealed that the occlusion-derived virus can be produced in cells infected with the Bm59-knockout virus. These results indicated that Bm59 is an early gene and is not essential for viral replication or assembly of BmNPV. These findings suggested that non-essential gene (Bm59) remained in the viral genome, which may interact with other viral/host genes in a certain situation. Keywords Bombyx mori nucleopolyhedrovirus (BmNPV) Bm59 gene Early gene