Production of biomass and bioactive compounds by adventitious root suspension cultures of Morinda citrifolia (L.) in a liquid-phase airlift balloon-type bioreactor

详细信息    查看全文

• 作者：Md. Abdullahil Baque (1) (2)
  Md. Humayun Kabir Shiragi (3)
  Sang-Hyun Moh (4)
  Eun-Jung Lee (1) (5)
  Kee-Yoeup Paek (1)
  
• 关键词：Adventitious roots ; Anthraquinones ; Bioreactor culture ; Oxidative stress ; Phenylalanine ammonia lyase
• 刊名：In Vitro Cellular & Developmental Biology - Plant
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：49
• 期：6
• 页码：737-749
• 全文大小：
• 作者单位：Md. Abdullahil Baque (1) (2)
  Md. Humayun Kabir Shiragi (3)
  Sang-Hyun Moh (4)
  Eun-Jung Lee (1) (5)
  Kee-Yoeup Paek (1)
  
  1. Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, 361-763, Republic of Korea
  2. Department of Agronomy, Sher-e-Bangla Agricultural University, Dhaka, 1207, Bangladesh
  3. Central Laboratory, Soil Resource Development Institute (SRDI), Ministry of Agriculture, Govt. of Bangladesh, Farmgate, Dhaka, 1215, Bangladesh
  4. Anti-aging Research Institute, BIO-FD&C Co., Ltd., 510 ho, Smart Valley A, Songdo, Incheon, 406-840, Republic of Korea
  5. Cheongsol Biotech Co. Ltd., Industry Academic Cooperation Foundation Agribusiness Incubator Center 205, Chungbuk National University, Cheongju, 361-763, Republic of Korea
  
• ISSN：1475-2689

文摘

To improve root growth and production of bioactive compounds such as anthraquinones (AQ), phenolics, and flavonoids by adventitious root cultures of Morinda citrifolia, the effects of aeration rate, inoculum density, and Murashige and Skoog (MS) medium salt strengths were investigated using a balloon-type bubble bioreactor. The possible mechanisms underlying changes in activities of enzymic (superoxide dismutase, catalase, guaiacol peroxidase, ascorbate peroxidase) and nonenzymic (vitamin E) antioxidants, phenylalanine ammonia lyase, and stress levels (accumulation of hydrogen peroxide and proline, peroxidation of lipids) were also studied. Low aeration rate (0.05vvm [air volume/culture volume/min]) accelerated accumulation of root fresh weight and dry weight (DW). High aeration rates (0.1 to 0.3vvm) stimulated accumulation of AQ, phenolics, and flavonoids and reduced root growth. Low inoculum densities (5 and 10gl) increased accumulation of those metabolites but inhibited root growth. Culture of adventitious roots with high concentrations of MS salts (1and 1.5MS) resulted in induction of oxidative stress that strongly inhibited root growth. Overall, an aeration rate of 0.05vvm, 15gl inoculum density, and half-strength (0.5 MS medium were optimal for enhancing accumulation of root dry biomass (4.38gl), AQ (103.08mgg DW), phenolics (54.81mgg DW), and flavonoids (49.27mgg DW).